Purification kit for plant and fungi total RNA

The Plant/Fungi Total RNA Purification Kit from Norgen enables the rapid and efficient purification of total RNA, including microRNA, from plant and fungal samples.

Image Credit: Norgen Biotek Corporation

Features and benefits

• Extracts all RNA species, including viral and viroid RNA.
• Strong lysis buffer effectively processes even challenging samples such as pine needles and grape leaves.
• Isolates total RNA, including microRNA, without the use of phenol.
• Produces high-quality RNA with excellent integrity and diversity.
• Available in a 96-well format for high-throughput applications.
• Utilizes spin column chromatography based on Norgen’s proprietary resin separation matrix.

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Format

Spin column

Size

• 50 Preps
• 100 Preps
• 250 Preps

High throughput

Size

2 × 96-Well Plates

Norgen’s Plant/Fungi Total RNA Purification Kit offers a fast and reliable method for isolating total RNA—including viral and viroid RNA—from a broad range of plant sources. It’s suitable for use with fresh or frozen plant tissues, cultured plant cells, and filamentous fungi. The kit captures all RNA sizes, including small RNAs like microRNA (miRNA), through a straightforward and efficient protocol.

Importantly, the purification process avoids the use of phenol or chloroform, making it safer and more user-friendly. The resulting RNA is of high quality and well-suited for a variety of downstream applications, such as real-time PCR, reverse transcription PCR, Northern blotting, RNase protection assays, primer extension, and expression array analysis.

For labs handling large sample volumes, the kit is also available in a 96-well high-throughput format. RNA purification in this format can be carried out using either a vacuum manifold or standard centrifugation.

Details

Supporting data

Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen's kit and a competitors kit. Five microlitres of total RNA from the 50 µL elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Norgen's kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles, and strawberry. Furthermore, only Norgen's kit was able to isolate the small RNA species (white box). Image Credit: Norgen Biotek Corporation

Figure 2. Detection of EF1-α using one step RT-qPCR from Challenging Plant Samples. Total RNA was isolated from 50 mg samples of apple (red), peach (green), pine needle (blue), and grape leaves (burgundy) using Norgen's Plant/Fungi Total RNA Purification Kit. Three µL of the eluted 50 µL of RNA was used in an RT-qPCR reaction for the detection of EF1-α. EF1-α was detected from all samples, indicating that the RNA is of high quality and that the Plant/Fungi RNA Purification kit is highly sensitive for total RNA isolation. Image Credit: Norgen Biotek Corporation

Figure 3. High Yield of Plant RNA Samples isolated from Apple, Peach, and Pear. Total RNA was isolated using Norgen's kit (Blue bars) and a competitor’s kit (Dark blue bars) from 50 mg of apple, peach and pear according to the provided manual. In each case the RNA was eluted into 50 µL. RNA yield was measured by spectrophotometer in triplicate, and the average concentration was used for data analysis. Norgen's kit resulted in higher yields of total RNA, including miRNA. Total processing time was less with Norgen's kit than competitor's kit. Image Credit: Norgen Biotek Corporation

Figure 4. High Purity of RNA Samples Isolated from Apple, Peach, and Pear. RNA was isolated from 50 mg samples of apple, peach and pear using Norgen's Plant/Fungi RNA Purification Kit and a competitor's kit. RNA purity was determined spectrophotometrically for the RNA isolated using Norgen's kit (260/230 = dark blue bars) and the competitor's kit (260/230 = light blue bars). Also shown is the 260/280 ratio for the samples. Norgen's kit consistently isolated pure samples of Plant RNA with 260/230 ratios above 2.0, whereas the competitor's kit had 260/230 ratios below 1.2 for the same samples. Image Credit: Norgen Biotek Corporation

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Spin column

Source: Norgen Biotek Corporation

* Yield will vary depending on the type of sample processed.

High throughput

Source: Norgen Biotek Corporation

* Yield will vary depending on the type of sample processed.

Storage conditions and product stability

Every solution needs to be stored at room temperature and kept tightly sealed. This kit remains stable for two years following the date of shipment.

Select plants and fungi tested with the Norgen plant/fungi total RNA purification kits

Plants

• Pepper (Capsicum annuum)
• Tobacco (Nicotiana tabacum)
• Tomato (Lycopersicon esculentum)
• Peach (Prunus persica)
• Potato (Solanum tuberosum)
• Arabidopsis thaliana1
• Apple (Malus sp.)
• Pear (Pyrus sp.)
• Grape vine (Vitis sp.)
• Pine needle (Pinaceae)
• Plum (Prunus sp.)
• Palm (Arecaceae)
• Blackberry
• Strawberry
• Raspberry
• Potato tuber (Solanum)
• Herbs
• Persimmon (Ebenaceae)
• Plum fruit
• Cotton (Gossypium)
• Citrus
• Vanilla bean
• Grape berry skin
• Mangrove
• Chrysanthemum
• Eastern White Red Cedar
• Kiwi leaves
• Peach (fruits and flowers)
• Soy bean (legume)
• Corn leaves
• Cucumber leaves

Fungi

• Cladosporium cladosporioides
• Aspergillus niger
• Alternaria tenuissima
• Mucor racemosus
• Fusarium oxysporum
• Penicillium sp.
• Pichia sp.
• Botrytis cinerea (Botryotinia fuckeliana)
• Rhizopus oryzae

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Source: Norgen Biotek Corporation