High-quality RNA isolation from purified exosomes

A fast and simple protocol for isolating exosomal RNA from exosomes previously purified using Norgen's Exosome Purification Kits, ultracentrifugation, size-exclusion chromatography, or most other commercial methods.

Image Credit: Norgen Biotek Corporation

Image Credit: Norgen Biotek Corporation

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Format

Spin Column

Size

Variable

Features and benefits:

• Norgen’s proprietary resin is used in a spin column chromatography process for purification
• Purify high-quality RNA in 15-20 minutes
• Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
• No Proteinase K treatment
• Bind and elute all RNA irrespective of size or GC content, without bias
• No phenol extractions
• No carrier RNA
• Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
• The isolated RNA is compatible with numerous downstream applications, notably Small RNA Sequencing.

This kit offers a fast, reliable, and convenient method for isolating and concentrating exosomal RNA from previously purified exosomes. It enables RNA extraction from intact extracellular vesicles (EVs) obtained from various urine or plasma/serum sample volumes, utilizing Norgen’s proprietary resin for purification.

The Exosomal RNA Isolation Kit is designed to capture RNA of all sizes, including microRNA. It provides a flexible elution volume ranging from 50 µL to 100 µL, ensuring high-integrity RNA suitable for multiple downstream applications such as real-time PCR, reverse transcription PCR, Northern blotting, primer extension, RNase protection assays, and expression array analysis.

Details

Supporting data

Figure 1. Isolation of RNA from exosomes purified from different urine volumes. RNA from exosomes isolated from various urine volumes purified using Norgen's Urine Exosome Purification Kits (Cat# 57700, 57800, and 57900) was isolated using Norgen's Exosomal RNA Isolation Kit (Cat# 58000). The amplification of the isolated urinary exosomal miR-30a was then evaluated using RT-qPCR reactions with 2 µL of the extracted RNA as the template. (A) As the sample input volume increases, the average Ct value for urinary exosomal miR-30a decreases linearly. B) With a recovery rate of over 90%, the relative amount of urinary exosomal miR-30a exhibits good linearity. Image Credit: Norgen Biotek Corporation

Figure 2. Isolation of RNA from exosomes purified from different plasma volumes. RNA from exosomes isolated from various plasma volumes purified using Norgen's Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500, and 57600) was isolated using the company's Exosomal RNA Isolation Kit (Cat# 58000). The amplification of the isolated plasma exosomal miR-26a was then evaluated using RT-qPCR reactions with 2 µL of the extracted RNA as the template. (A) As the sample input volume increases, the average Ct value for plasma exosomal miR-26a decreases linearly. B) With a recovery percentage of over 90%, the relative amount of plasma exosomal miR-26a exhibits outstanding linearity. Image Credit: Norgen Biotek Corporation

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Kit specifications

Source: Norgen Biotek Corporation

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage conditions and product stability

Every buffer needs to be stored at room temperature and kept tightly sealed. This kit remains stable for two years following the date of shipment.

If any salt precipitation is seen, Lysis Buffer A should be warmed for 20 minutes at 60 °C.

Source: Norgen Biotek Corporation