The Soil DNA Isolation Maxi Kit from Norgen allows for the detection of microorganisms from large soil samples.
Features and benefits
- A rapid and convenient method for the detection of microorganisms in up to 10 g of soil samples
- Efficiently remove organic substances using the OSR Solution
- Fast and easy processing using a rapid spin-column format
- Isolate high-quality total DNA from a variety of microorganisms, including bacteria, fungi, and algae
- Process all soil types
- Remove all humic acid from DNA samples using the Maxi Humic Acid Removal Columns
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Format
Maxi
Size
10 Preps
Norgen’s Soil DNA Isolation Maxi Kit offers a fast and user-friendly solution for extracting DNA from large soil samples—up to 10 grams. It’s designed to handle a wide range of soil types, from standard field samples to more challenging ones rich in humic substances, like compost and manure.
The kit effectively removes humic acids using a dedicated Humic Acid Removal Column alongside the OSR (Organic Substance Removal) Solution. Following this step, a straightforward spin column procedure further purifies the DNA. The result is high-quality total genomic DNA from a broad spectrum of soil microorganisms, including bacteria, fungi, and algae.
The purified DNA is of the highest quality and is fully compatible with downstream PCR and NGS applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Details
Supporting data
Figure 1. Resolution of DNA isolated from 10 g of two different soil types; regular soil (clay) and high humic acid soil (top soil) using Norgen’s Soil DNA Isolation Maxi Kit. For evaluation, 10 µL from the 1st elution (2 mL) and 2nd elution (2 mL) from each soil sample was run on 1× TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Soil DNA Isolation Maxi Kit. Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900). Image Credit: Norgen Biotek Corporation
Figure 2. Difference in DNA yield between two soil types (clay and top soil) and elutions (1st and 2nd elution). Ten grams of soil was processed using Norgen’s Soil DNA Isolation Maxi Kit. The second elution increased the total DNA yield by an additional 20-30%. Image Credit: Norgen Biotek Corporation
Figure 3. High soil DNA quality was confirmed by real-time PCR using 8 µL of soil DNA (total PCR reaction volume was 20 µL) isolated using Norgen’s Soil DNA Isolation Maxi Kit to detect 16 s rDNA. The 16 s rRNA gene was successfully amplified in both the first elution (red) and second elution (blue) from two soil types (Clay = circle, Top soil = diamond) without PCR inhibition, indicating the excellent soil DNA quality from 10 grams of soil. Image Credit: Norgen Biotek Corporation
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Kit specifications
Source: Norgen Biotek Corporation
| Kit specifications |
| Maximum Soil Input |
10 g |
| Type of Soil Processed |
All soil types |
| Maximum Column Binding Capacity |
1.5 mg |
| Average Yield from 10 g of soil* |
30-50 μg |
| Time to Complete 10 Purifications |
35 minutes (1 hour with OSR steps) |
* Average yields will vary depending on soil sample types
Storage conditions and product stability
All solutions should be tightly sealed and stored at room temperature. When stored properly, the kit remains stable for up to one year from the date of shipment.
Source: Norgen Biotek Corporation
| Component |
Cat. 62000 (10 preps) |
| Lysis Buffer G |
2 x 100 mL |
| Lysis Additive A |
25 mL |
| Binding Buffer I |
25 mL |
| OSR Solution |
12 mL |
| Buffer SK |
2 x 60 mL |
| Wash Solution A |
1 x 18 mL 3 x 38 mL |
| Elution Buffer B |
30 mL |
| Maxi Bead Tubes |
10 |
| Humic Acid Removal Columns |
10 |
| Maxi Spin Columns |
10 |
| Elution Tubes |
10 |
| Product Insert |
1 |