Stem Cell Factor (SCF), also known as kit-ligand (KL), steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SF, or SHEP7, is a cytokine that binds to the c-Kit receptor (CD117). SCF exists in both transmembrane and soluble forms, generated through alternative splicing of the same RNA transcript. It is crucial in hematopoiesis (blood cell formation), spermatogenesis, and melanogenesis.
Fibroblasts and endothelial cells produce forms of SCF, with the soluble version having a molecular weight of 18.5 kDa and forming a dimer. SCF is essential for hematopoiesis during embryonic development, with key hematopoietic sites such as the fetal liver and bone marrow expressing this cytokine. SCF also influences the localization of melanocytes, the pigment-producing cells responsible for skin and hair coloration.
In the adult bone marrow, SCF plays a regulatory role in the hematopoietic stem cell (HSC) niche. It is often combined with other cytokines to culture HSCs and hematopoietic progenitors. The ability to expand these cells ex vivo presents significant potential for advancements in bone marrow transplantation, enabling the re-establishment of blood cell formation in patients.
Features
- Developed according to ISO 9001:2015 and ISO 13485:2016
- Produced and tested for quality control in a GMP-compliant facility
- Free from beta-lactam materials
- Free from animal-derived materials
- Consistent performance across batches
- Rigorous quality control testing
Source
GMP Human SCF Protein (GMP-SCFH25) is expressed in human 293 cells (HEK293) and includes amino acids Glu 26 to Ala 189 (Accession # P21583-1).
Predicted N-terminus: Glu 26
Molecular characterization
Image Credit: ACROBiosystems Inc
This protein has no "tags."
The protein has an approximate molecular weight of 18.5 kDa. Due to glycosylation, the protein moves as a 30 kDa ± 3 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 10 EU/mg endotoxin by the LAL method.
Host cell protein
<0.5 ng/µg of protein as identified by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Sterility testing was carried out using the membrane filtration method, as outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1.
Mycoplasma
Negative
Purity
>95% quantified by SDS-PAGE
Formulation
The protein is lyophilized from a 0.22 μm filtered PBS solution at pH 7.4 with protectants.
Shipping
This product is delivered with blue ice during shipping.
Storage
Upon arrival, store immediately at -20 °C or below for long-term preservation.
Avoid multiple freeze-thaw cycles.
This product is stable after storage at:
- -70 °C for 12 months under sterile conditions after reconstitution
- -20 °C to -70 °C for five years in lyophilized state
ACRO quality management system
- Quality Advantages
- QMS（ISO, GMP)
- Quality Control Process
QC and assay protocol
SDS-PAGE
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GMP Human SCF Protein analyzed by SDS-PAGE under reducing (R) conditions, with Coomassie Blue staining, shows a purity exceeding 95%.
Bioactivity-Bioactivity CELL BASE
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GMP Human SCF Protein (Cat. No. GMP-SCFH25) promotes the proliferation of the Mo7e cell line. Its specific activity is > 5.00 × 10⁵ IU/mg, calibrated against the human SCF WHO International Standard (NIBSC code: 91/682) (QC tested).
Application data
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GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF basic Protein (Cat. No. GMP-FGCH17), and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) significantly enhance iPSC differentiation into HSPCs after 14 days, leading to high expression of hematopoietic stem cell markers CD34 and CD45.
Image Credit: ACROBiosystems Inc
GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18), and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) support the rapid expansion and high viability of CD34+ hematopoietic stem cells.
Bioactivity-Stability
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Cell-based assays confirm that GMP Human SCF Protein (Cat. No. GMP-SCFH25) remains stable at 4 °C for 180 days.
Image Credit: ACROBiosystems Inc
The cell-based assay demonstrates that GMP Human SCF Protein (Cat. No. GMP-SCFH25) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc
The cell-based assay shows that GMP Human SCF (Cat. No. GMP-SCFH25) maintains stability thrice after freezing and thawing.
Image Credit: ACROBiosystems Inc
The cell-based assay reveals batch-to-batch consistency between Acro's GMP and PG SCF.