Interleukin 12 (IL-12), also known as p70, is a cytokine naturally produced by dendritic cells, macrophages, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. It is a heterodimeric molecule composed of two subunits, IL-12A (p35) and IL-12B (p40).
IL-12 plays a key role in the immune response by promoting the differentiation of naive T cells into Th1 cells. As a T cell-stimulating factor, it enhances the growth and function of T cells while inducing the production of IFN-γ and TNF-α from both T cells and natural killer (NK) cells. Additionally, IL-12 counteracts IL-4-mediated suppression of IFN-γ, further supporting immune activation.
Beyond its immunomodulatory functions, IL-12 is also involved in anti-angiogenic processes, inhibiting the formation of new blood vessels. This property has potential implications for cancer therapy and other critical angiogenesis conditions.
Features
- Developed in accordance with ISO 9001:2015 and ISO 13485:2016 standards
- Produced and quality control (QC) tested in a GMP-compliant facility
- Composed of animal-free materials
- Free from beta-lactam components
- Ensures batch-to-batch consistency
- Undergoes rigorous quality control testing
Source
GMP Human IL-12 Protein (GMP-L12H23) is derived from human 293 cells (HEK293). It includes amino acids Ile 23 - Ser 328 and Arg 23 - Ser 219 (Accession # P29460-1 & P29459-1).
Predicted N-terminal sequence: Ile 23
Molecular characterization
- This protein is produced without any “tag.”
- The calculated molecular weight (MW) is 58.9 kDa. When analyzed under reducing conditions (SDS-PAGE) and calibrated against the Star Ribbon Pre-stained Protein Marker, the protein migrates as 76 kDa ± 3 kDa due to glycosylation.
Endotoxin
Contains less than 10 EU/mg as determined by the LAL method.
Host cell protein
Less than 0.5 ng/µg of protein, as tested by ELISA.
Host cell DNA
Less than 0.02 ng/μg of protein, as determined by qPCR.
Sterility
Sterility testing is conducted using the membrane filtration method, following CP<1101>, USP<71>, and Eur. Ph. 2.6.1 guidelines.
Mycoplasma
Negative
Purity
Greater than 95% purity, as confirmed by SDS-PAGE.
Formulation
Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with added protectants.
Shipping
The product is shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term preservation. Avoid repeated freeze-thaw cycles.
Stability:
- Lyophilized state: Stable for 5 years when stored at -20 °C to -70 °C.
- After reconstitution under sterile conditions: Stable for 12 months at -70 °C.
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Comprehensive Quality Control Process
QC and assay protocol
SDS-PAGE
SDS-PAGE analysis of GMP Human IL-12 Protein in reducing (R) and non-reducing (NR) conditions. Coomassie Blue was used to stain the gel. According to Star Ribbon Pre-stained Protein Marker, the protein's purity is higher than 95%.
Image Credit: ACROBiosystems Inc
Bioactivity-CELL BASE
The GMP Human IL-12 Protein (Cat. No. GMP-L12H23) causes NK-92 to secrete more IFN-γ. When compared to the human IL-12 WHO International Standard (NIBSC code: 95/544), the specific activity of GMP Human IL-12 Protein is > 1.00 ⅹ 107 IU/mg (QC tested).
Image Credit: ACROBiosystems Inc
Compared to competing products, GMP Human IL-12 Protein (Cat. No. GMP-L12H23) exhibited a higher activity level.
Image Credit: ACROBiosystems Inc
Application data
For two weeks, human PBMCs were cultivated in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-12 Protein (ACROBiosystems, Cat. No. GMP-L12H23), and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26).
According to the results, GMP Human IL-12 Protein (ACROBiosystems) can support these cells' growth while maintaining a respectable level of cell viability and can be compared to the competitor M IL-12 protein.
Image Credit: ACROBiosystems Inc
For two weeks, human PBMCs were cultivated in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-12 Protein (ACROBiosystems, Cat. No. GMP-L12H23), and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26).
The outcome demonstrates that the competitor M IL-12 protein and GMP Human IL-12 Protein (ACROBiosystems) can be compared.
Image Credit: ACROBiosystems Inc
Bioactivity-Stability
According to the cell-based assay, the GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc
The GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable at 4 °C for 180 days, according to the cell-based assay.
Image Credit: ACROBiosystems Inc
GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable after three freezing and thawing cycles, according to the cell-based assay.
Image Credit: ACROBiosystems Inc
Batch-to-batch consistency between PG IL-12 and Acro's GMP is demonstrated by the cell-based assay.
Image Credit: ACROBiosystems Inc