Delta-like protein 4 (DLL4), also known as Drosophila Delta homolog 4 (Delta4), contains one DSL domain and eight EGF-like domains. It is expressed in vascular endothelium and functions as a Notch ligand, activating NOTCH1 and NOTCH4. DLL4 plays a role in angiogenesis by negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. It binds to both Notch-1 and Notch-4.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
Source
GMP Human DLL4 Protein, Fc Tag (GMP-DL4H28) is expressed in human 293 cells (HEK293) and consists of amino acids Ser27–Pro524 (Accession # NP_061947.1).
Predicted N-terminus: Ser 27
Molecular characterization
Image Credit: ACROBiosystems
This protein carries a human IgG1 Fc tag at the C-terminus.
It has a calculated molecular weight of 80.7 kDa but migrates at approximately 90 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE due to glycosylation.
Endotoxin
The LAL technique yields less than ten EU/mg.
Protein A
<5 ppm of protein tested by ELISA.
Host cell protein
<0.5 ng/µg of protein tested by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative
Purity
>95 % as determined by SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is delivered and transported with blue ice.
Storage
Store it at -20 °C or lower for long-term storage as soon as it arrives.
Avoid repeating the freeze-thaw cycle.
Following storage, this product remains stable at:
- -20 °C to -70 °C in a lyophilized form for five years
- Following reconstitution, store at -70 °C for 12 months under sterile circumstances.
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
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GMP Human DLL4 Protein, Fc Tag was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 %.
Bioactivity-CELL BASE
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GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) stimulates Human Notch1 (Luc) HeLa Reporter Cells, with an EC50 of 9.10 µg/mL (routinely tested).
Bioactivity-SPR
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GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) captured on a Protein A chip binds to Human NOTCH1 Protein, His Tag, Premium Grade (Cat. No. NO1-H52H3) with an affinity constant of 1.00 nM – 150 nM, as determined by SPR assay (Biacore 8K) (QC tested).
Application data
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CD34+ CD45+ hematopoietic cells were seeded on GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28)-coated plates and differentiated for 26 days. Flow cytometry analysis detected the expression of T-cell progenitor markers CD7 and CD5. In combination with other growth factors, GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) induced the formation of CD7+ and CD7+ CD5+ T-cell progenitors. In contrast, cells cultured without GMP Human DLL4 Protein, Fc Tag-coated plates did not express CD5 or CD7.
Image Credit: ACROBiosystems
The Recombinant Fibronectin Fragment, Premium Grade (Cat. No. FIN-H5116), combined with GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25), efficiently induced hematopoietic stem cell differentiation into NK cells, characterized by high expression of CD56+ CD3-.
Bioactivity-Stability
Image Credit: ACROBiosystems
GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) is stable at 37 °C for 24 hours, according to the SPR-based assay.
Image Credit: ACROBiosystems
After three freezing and thawing cycles, the SPR-based assay demonstrates that the GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) remains stable.