FGF basic is a member of the FGF family, which includes at least 23 related mitogenic proteins sharing 35–60% amino acid conservation. Unlike most other FGFs, FGF acidic and FGF basic lack signal peptides and are secreted through non-classical pathways.
FGF basic has been isolated from various sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues; however, its biological activity remains intact even in its reduced form, indicating that disulfide bonds are not essential for function.
bFGF is a key component of human embryonic stem cell culture media, required to maintain cells in an undifferentiated state, though the exact mechanisms remain unclear. It has been shown to induce gremlin expression, which inhibits differentiation triggered by bone morphogenetic proteins. bFGF is necessary in both mouse feeder cell-dependent culture systems and feeder- and serum-free culture conditions.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
- No animal-derived peptone or lactose used in the production process
Source
GMP Human FGF Basic Protein (GMP-FGCH17) is expressed in E. coli cells and consists of amino acids Pro143–Ser288 (Accession # P09038-4).
Predicted N-terminus: Pro 143
Molecular characterization
Image Credit: ACROBiosystems
This protein carries no tag.
It has a calculated molecular weight of 16.5 kDa but migrates at approximately 17 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE.
Endotoxin
The LAL technique yielded less than 10 EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
Sterile testing was carried out using the membrane filtering method as outlined in Eur. Ph. 2.6.1, USP<71>, and CP<1101>.
Mycoplasma
Negative
Purity
>95 %, according to SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
Blue ice is used to package and ship this product.
Storage
Store it at -20 °C or lower for long-term storage as soon as it is delivered.
Avoid repeating the freeze-thaw cycle.
This product remains stable after storage at:
- -20 °C to -70 °C for 5 years in the lyophilized condition
- Following reconstitution, store at -70 °C for 12 months under sterile circumstances.
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Human FGF Basic Protein was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 %.
Bioactivity-Bioactivity CELL BASE
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GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17) stimulates the proliferation of NIH/3T3 cells. Its specific activity is >2.50 × 106 IU/mg, calibrated against the Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712) (QC tested).
Image Credit: ACROBiosystems
The activity of GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17) is higher than that of competing products.
Application data
Left: The mRNA expression of pluripotent stem cell markers (Oct4, Nanog) was higher in Acro than in company R and company P. Right: When FGF basic (35 μg/mL) is added into the culture media (StemSure hPSC medium) for iPSC, the cells remain high expression of pluripotent (Oct4+ or Nanog+; pink) markers. Image Credit: ACROBiosystems
FGF basic (Cat. No. GMP-FGCH17) can offer greater support for stemness maintenance in ESC/iPSCs than other companies.
Image Credit: ACROBiosystems
GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17), and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) significantly promoted iPSC differentiation into HSPCs after 14 days, resulting in high expression of hematopoietic stem cell markers CD34 and CD45.
Image Credit: ACROBiosystems
GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17) and GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25), in combination with GMP Human Laminin 521 Protein (Cat. No. GMP-LA5H24), effectively maintain the stemness of hiPSCs, ensuring high expression of stem cell markers OCT4, SOX2, and SSEA4.
Bioactivity-Stability
Image Credit: ACROBiosystems
The Cell-based assay demonstrates that GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17) remains stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems
Following three cycles of freezing and thawing, the cell-based assay demonstrates that GMP Human FGF basic Protein (Cat. No. GMP-FGCH17) remains stable.
Image Credit: ACROBiosystems
According to the cell-based assay, the GMP Human FGF basic Protein (Cat. No. GMP-FGCH17) is stable at 4 °C for 30 days.
Image Credit: ACROBiosystems
The cell-based assay shows batch-to-batch consistency between Acro's GMP and PG FGF basic.