CD3e molecule, epsilon (CD3E), is a single-pass type I membrane glycoprotein found on T cells. It contains one Ig-like (immunoglobulin-like) domain and one ITAM domain.
CD3E, along with CD3-gamma, CD3-delta, CD3-zeta, and T-cell receptor (TCR) alpha/beta or gamma/delta heterodimers, forms the TCR-CD3 complex. This complex is crucial for coupling antigen recognition to intracellular signal transduction pathways.
The genes encoding CD3E, CD3G (gamma), and CD3D (delta) polypeptides are clustered on chromosome 11. CD3E plays a critical role in T-cell development, and mutations in the CD3E gene can lead to severe immunodeficiency. Additionally, CD3E has been linked to susceptibility to type I diabetes in women.
CD3E has been shown to interact with TOP2B, CD3EAP, and NCK2.
Introduction
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Animal-free materials
- Consistent batch-to-batch quality
- Stringent quality control testing
Source
Human GMP Monoclonal Anti-Human CD3 Antibody (OKT3) is expressed in HEK293 cells.
Isotype
Mouse IgG2a/kappa.
Conjugate
Unconjugated.
Specificity
This antibody specifically binds to CD3 epsilon.
Endotoxin
Contains less than 2 EU/mg as determined by the LAL method.
Protein A
Protein A content is less than 5 ppm, verified by ELISA.
Host cell protein
Host cell protein content is less than 0.5 ng/μg of protein, tested by ELISA.
Host cell DNA
Host cell DNA content is less than 0.02 ng/μg of protein, tested by qPCR.
Purity
- Greater than 95 % purity as determined by SDS-PAGE.
- Greater than 95 % purity as determined by SEC-HPLC.
Sterility
Sterility testing is conducted using the membrane filtration method as described in CP<1101>, USP<71>, and Ph. Eur. 2.6.1.
Mycoplasma
Tested negative for mycoplasma contamination.
Formulation
Provided as a 0.2 μm filtered solution in PBS, pH 7.4, with added protectants.
Shipping
Shipped with dry ice.
Storage
The product must be kept in a liquid state at -70 °C for extended periods of time.
Once stored, this product is stable for:
- 12 months at 2–8 °C in sterile conditions
- Five years at -70 °C
ACRO quality management system
- QMS (ISO, GMP).
- Quality Advantages
- Comprehensive quality control process.
QC and assay protocol
SDS-PAGE
GMP Monoclonal Anti-Human CD3 Antibody (OKT3) was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming that the protein's purity exceeds 95 %.
Image Credit: ACROBiosystems
SEC-HPLC
The purity of GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) is greater than undefined, and the molecular weight of this protein is approximately undefined, as verified by SEC-MALS.
Image Credit: ACROBiosystems
Bioactivity-Bioactivity CELL BASE
GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) stimulates IL-2 secretion in PBMCs when co-stimulated with 10 ng/mL Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The typical EC50 for this effect is 0.11 ng/mL (QC tested).
Image Credit: ACROBiosystems
The activity of GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) is higher than that of competing products.
Image Credit: ACROBiosystems
Bioactivity-Stability
At 37 °C, GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) remains stable for seven days, according to the cell-based assay.
Image Credit: ACROBiosystems
According to the cell-based assay, GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) remains stable following three cycles of freezing and thawing.
Image Credit: ACROBiosystems
ACRO's GMP and PG CD3 Antibody exhibit batch-to-batch consistency in the cell-based assay.
Image Credit: ACROBiosystems