T-cell-specific surface glycoprotein CD28, also known as TP44, is a single-pass type I membrane protein that contains one Ig-like V-type (immunoglobulin-like) domain. It is a key co-stimulatory molecule required for T cell activation.
CD28 serves as the receptor for CD80 (B7.1) and CD86 (B7.2). When antigen-presenting cells (APCs) are activated by Toll-like receptor ligands, CD80 expression is upregulated, while CD86 expression remains constitutive. Notably, CD28 is the only B7 receptor constitutively expressed on naïve T cells.
Introduction
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Animal-free materials
- Consistent batch-to-batch quality
- Stringent quality control testing
Source
Human GMP Monoclonal Anti-Human CD28 Antibody is expressed in HEK293 cells.
Isotype
Mouse IgG1/Kappa
Conjugate
Unconjugated
Specificity
This product is a specific antibody that selectively reacts with CD28.
Endotoxin
The LAL method yielded less than 2 EU/mg.
Protein A
ELISA test for protein levels <5 ppm.
Host cell protein
Protein tested by ELISA: <0.5 ng/μg.
Host cell DNA
Protein tested by qPCR was less than 0.02 ng/μg.
Purity
According to SDS-PAGE, 95 %.
>95 %, as SEC-HPLC measured
Sterility
According to CP<1101>, USP<71>, and Ph. Eur. 2.6.1, the membrane filtration method was used to conduct the sterility test.
Mycoplasma
Negative
Formulation
Provided in the form of a 0.2 μm filtered solution in PBS, polysorbate 80, pH 7.4, and protective agents.
Shipping
This product is supplied and shipped with dry ice.
Storage
The product should be kept at -70 °C in a liquid state for extended periods of time.
Following storage, this product remains stable at:
- 2–8 °C for 12 months under sterile conditions
- -70 °C for 5 years
ACRO quality management system
- GMP, ISO, and QMS
- Benefits of Quality
- Procedure for Quality Control
QC and assay protocol
SDS-PAGE
GMP Monoclonal Anti-Human CD28 Antibody was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming that the protein's purity exceeds 95 %.
Image Credit: ACROBiosystems
SEC-HPLC
The purity of GMP Monoclonal Anti-Human CD28 Antibody (Cat. No. GMP-MC2824) exceeds an unspecified threshold, and its molecular weight is approximately undefined, as determined by SEC-MALS.
Image Credit: ACROBiosystems
Bioactivity-Bioactivity CELL BASE
When PBMC are stimulated with 4 ng/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3), GMP Monoclonal Anti-Human CD28 Antibody (Cat. No. GMP-MC2824) causes them to secrete more IL-2. According to QC testing, this effect's typical EC50 is 1.382 ng/mL.
Image Credit: ACROBiosystems
GMP Monoclonal Anti-Human CD28 Antibody (Cat. No. GMP-MC2824) exhibited greater activity than other competing products.
Image Credit: ACROBiosystems
Bioactivity-Stability
According to the cell-based assay, GMP Monoclonal Anti-Human CD28 Antibody (Cat. No. GMP-MC2824) remains stable for seven days at 37 °C for 7 days.
Image Credit: ACROBiosystems
Following three cycles of freezing and thawing, the cell-based assay demonstrates that the GMP Monoclonal Anti-Human CD28 Antibody (Cat. No. GMP-MC2824) remains stable.
Image Credit: ACROBiosystems
The cell-based assay shows batch-to-batch consistency between Acro's GMP and PG CD28 Antibody.
Image Credit: ACROBiosystems