Sino Biological has a vast amount of experience in the development and production of custom antibodies, providing high-quality antibody production services to research institutions, pharmaceutical enterprises, and diagnostic companies, supporting basic research, biological drug development, diagnostic reagent development, and other applications.
Sino Biological provides a range of quick antibody production services, such as fast rabbit polyclonal antibody production, fast mouse monoclonal antibody production, and beacon® antibody production service.
With the help of improved immunization protocols, Sino Biological has shortened the immunization period for the fast mouse mAb service from 8-12 weeks down to only 4-5 weeks, with the monoclonal antibodies being delivered in as little as 60 days.
The fast rabbit polyclonal antibody production service completes the antibody production process within 45 days, from animal immunization to purified antibody, while still delivering rabbit polyclonal antibodies with high ELISA titers (above 1:256,000).
Beacon® single-cell platform achieves positive clones in as few as 35 days, thereby ensuring that users have quick access to high-quality and affordable antibody production.
Service highlights
- Multi-platform assistance for quick antibody production
- A professional technical team with over 15 years of experience in antibody production
- Quick and improved animal immunization protocols
- Extensive range of validation platforms, such as WB, ELISA, IHC, and FACS
Fast antibody production packages
Fast mouse mAb production service
As fast as 60 days
Service details
- Antigen validation
- Fast immunization and serum titer test
- Pre-immune bleed
- 5 Balb/c mice immunization
- Serum titer test
- Final bleed
- Fusion and screening
- Fusion and screening
- Subcloning and cell expansion
- Cell banking
- Antibody production and QC analysis
- Hybridoma cell culture at the desired scale
- Protein A purification
- SDS-PAGE and UV analysis
- ELISA validation
Deliverables
- Around 2 to 5 ELISA positive clones (2 tubes cells or clone)
- CoA
- Purified antibodies, 0.1-0.5 mg/clone
- Remaining antigens
Guarantee
- An ELISA-positive outcome against the immunogen.
Note
- The antigen can either be provided by the client - or the client can use Sino Biological's antigen production service.
Add-on services
- Hybridoma Sequencing Service
- Hybridoma Antibody Production Service
Fast rabbit pAb production service
As fast as 45 days
Service details
- Antigen validation (1-2 days)
- Fast immunization and serum titer test
- Pre-immune bleed
- Two rabbits immunization
- Serum titer test
- Final bleed
- Purification (Optional) (3 days)
- Protein A purification
- Antigen affinity purification (If requested)
- QC analysis (3 days)
- SDS-PAGE and UV analysis
- ELISA validation
Deliverables
- Pre-immune sera, 20.5 mL/rabbit
- CoA
- Remaining antigens
- Antisera or purified antibodies
Guarantee
Note
- The antigen can either be provided by the client - or the client can use Sino Biological's antigen production service.
Beacon® mAb production service
As fast as 35 days
Service details
- Antigen validation (2-3 days)
- Fast Animal immunization (2-3 weeks)
- Multiple rounds of immunization
- ELISA-based titer test
- FACS-based titer test (optional)
- Beacon screening (1 day)
- Antigen-specific binding-based assays
- Cell-based antigen-specific binding assay
- Cross-species detection (optional)
- Ligand blocking assay (optional)
- Vector construction (1-2 weeks)
- Sequencing and vector construction
- Antibody expression and identification (2-3 weeks)
- Recombinant expression and QC analysis
Deliverables
- Antibody heavy and light chain sequences
- CoA
- Purified antibodies offered
Note
- The antigen can either be provided by the client - or the client can use Sino Biological's antigen production service.
Customer case study: Immunofluorescence antibodies generated by rapid immunization
Rapid immunization strategies have the potential to greatly decrease immunization time, which offers strong assistance for the development of some urgent projects.
Without increasing the dosage of immunogens, Sino Biological’s quick immunization platform is able to guarantee screening success rates and good animal immune responses and has successfully produced monoclonal antibodies for a range of applications like ELISA, WB, and IF.
In this project, recombinant HER2 protein was utilized as an immunogen for quick immunization of mice, and around nine immunofluorescence-positive antibodies were successfully generated.
The following was the assay outcome of one antibody, which displayed good sensitivity and specificity for immunofluorescence assays also in the hybridoma supernatant detection stage.
SKBR3. Image Credit: Sino Biological US Inc
MDA-MB-453. Image Credit: Sino Biological US Inc
MDA-MB-231 (Negative Control). Image Credit: Sino Biological US Inc
Note: SKBR3 (human breast cancer cell line), MDA-MB-453 (human breast cancer cell line), MDA-MB-231 (triple-negative breast cancer cell line).