The recurrent β chain signaling cytokines — granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 and IL-5 — can control the growth, activities and differentiation of eosinophils and other hematopoietic cells.
Therapeutic approaches to control such cytokines have been approved for use in several cancers as well as allergic and autoimmune diseases, while others exhibit potential early clinical activity.
Generally, cytokine heterodimeric receptors consist of two or three subunits: a significant ligand-binding subunit (the α subunit) and a signaling subunit (the β or γ subunit). This is epitomized by the common β-chain (CD131/ βc) of GM-CSF, IL-3 and IL-5 receptors; the gp130 (shared by the receptors for IL-6, IL-11), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), oncostatin M (OSM) and cardiotrophin; and the common IL-2 receptor γ-chain (shared by the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors).
Sino Biological has come up with a series of bioreagents to aid the study of β common chain cytokines and other significant cytokines and receptors.
βc family cytokines and receptors
The high-activity cytokine and receptor proteins from Sino Biological are obtained from several species and help study the biology of common β chain signaling cytokines.
Mouse IL-3 Protein, Tag Free — 51066-MNAH. Cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. Image Credit: Sino Biological Inc.
Human IL-5 Protein, N-cleavage — 5673-HNCE. Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.
Human GM-CSF Protein — 10015-HNAH. Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.
Human CSF2RB/CD131 Protein — 10516-H08H. Binding ability by ELISA. Immobilized human CD131 at 10 μg/mL (100 μL/well) can bind human Fc-GMCSF (Cat: 10015-H01H). Image Credit: Sino Biological Inc.
Recommended βc family cytokine and receptor proteins
IL-3 and IL-3 receptor proteins
Table 1. Source: Sino Biological Inc.
| Catalog |
Species |
Molecule |
Tag |
Expression Host |
Activity |
| 11858-H08H |
Human |
IL-3 |
C-His |
HEK293 Cells |
Active |
| 11858-HNAE |
Human |
IL-3 |
Native |
E. coli |
Active |
| 51066-MNAH |
Mouse |
IL-3 |
Native |
HEK293 Cells |
Active |
| 80235-R08B |
Rat |
IL-3 |
C-His |
Insect Cells |
Active |
| 10518-H02H |
Human |
IL3Ra |
C-hFc |
HEK293 Cells |
|
| 10518-H08H |
Human |
IL3Ra |
C-His |
HEK293 Cells |
|
| 50810-M02H |
Mouse |
IL3Ra |
C-hFc |
HEK293 Cells |
|
| 50810-M08H |
Mouse |
IL3Ra |
C-His |
HEK293 Cells |
|
| 70077-D08H |
Canine |
IL3Ra |
C-His |
HEK293 Cells |
|
IL-5 and IL-5 receptor proteins
Table 2. Source: Sino Biological Inc.
| Catalog |
Species |
Molecule |
Tag |
Expression Host |
Activity |
| 15673-HNAH |
Human |
IL-5 |
Native |
HEK293 Cells |
|
| 15673-HNCE |
Human |
IL-5 |
N-cleavage |
E. coli |
Active |
| 70019-D08B |
Canine |
IL-5 |
C-His |
Insect Cells |
Active |
| 51154-M08B |
Mouse |
IL5RA |
C-His |
Insect Cells |
Active |
GM-CSF and receptor proteins
Table 3. Source: Sino Biological Inc.
| Catalog |
Species |
Molecule |
Tag |
Expression Host |
Activity |
| 10015-H07H |
Human |
GM-CSF/CSF2 |
N-His |
HEK293 Cells |
Active |
| 10015-HNAH-B |
Human |
GM-CSF/CSF2 |
Native |
HEK293 Cells |
|
| 51048-M01H |
Mouse |
GM-CSF/CSF2 |
N-hFc |
HEK293 Cells |
Active |
| 51048-M07H |
Mouse |
GM-CSF/CSF2 |
N-His |
HEK293 Cells |
Active |
| 51048-MNAH |
Mouse |
GM-CSF/CSF2 |
Native |
HEK293 Cells |
Active |
| 80020-R01H |
Rat |
GM-CSF/CSF2 |
N-hFc |
HEK293 Cells |
Active |
| 80020-R07H |
Rat |
GM-CSF/CSF2 |
N-His |
HEK293 Cells |
Active |
| 10701-H02H |
Human |
CSF2RA |
C-hFc |
HEK293 Cells |
Active |
| 10701-H08H |
Human |
CSF2RA |
C-His |
HEK293 Cells |
Active |
| 10516-H08H |
Human |
CD131/CSF2RB |
C-His |
HEK293 Cells |
Active |
| 80323-R02H |
Rat |
CD131/CSF2RB |
C-hFc |
HEK293 Cells |
|
βc cytokine family antibodies
Sino Biological has come up with an extensive panel of antibodies that target the common β chain signaling cytokines and receptors. These antibodies can be utilized in FCM, ELISA, IHC, IP and WB. The company also offers human and mouse ELISA pair sets.
- Anti-IL3
- Anti-IL5
- Anti-IL3RA
- Anti-IL5RA
- Anti-GM-CSF
- Anti-CSF2RA/CD116
- Anti-CSF2RB/CD131