The pathogenesis of ischemia-reperfusion injury of heart, acute pancreatitis, neurodegeneration and brain and kidney, muscular dystrophies, have a common key characteristic - mitochondrial abnormality.
A drastic rise in permeability of inner mitochondrial membrane (IMM), through consistent opening of a multi-protein channel referred to as the mitochondrial permeability transition pore (MPTP) results in mitochondrial dysfunction.
Following this, uncontrolled proton flow through the IMM occurs along with unregulated transfer of ions, water, and small solutes across the mitochondrial matrix. Rupture of outer mitochondrial membrane (OMM) and ultimately cell death due to necrosis are the end results. Hence, for the prevention of cell death in a variety of disease conditions, the MPTP is a potential target.
The calcium retention capacity technique uses spikes of calcium ions to challenge the isolated mitochondria. After opening of MPTP, fluorescence of membrane-impermeable CalciumGreen™ dye is enhanced as Ca2+ leaches into the buffer. The Omega multi-mode plate reader has proven outstanding robustness for carrying out multiple injections. The device has also shown a reliable fluorescent recognition of the assay.
About BMG Labtech
BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.
BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.
All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.
Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.
Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.
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