Fungi/Yeast Genomic DNA Isolation Kits from Norgen are designed for the fast and efficient purification of DNA from yeast cells, fungal spores, or mycelium.
Image Credit: Norgen Biotek Corporation
Image Credit: Norgen Biotek Corporation
Image Credit: Norgen Biotek Corporation
Image Credit: Norgen Biotek Corporation
Image Credit: Norgen Biotek Corporation
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Features and benefits
- Bead tubes (provided) mean that effective mechanical homogenization can be carried out
- Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more
- Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria, including Gram-positive
Format
Spin column
50 Preps
High throughput
2 × 96-Well Plates
These kits offer fast, reliable methods for purifying genomic DNA from a range of microorganisms, including viable yeast cells, fungal spores or mycelium, and Gram-positive bacteria. DNA extraction is achieved through a combination of heat treatment, detergents, and Bead Tubes (included in the kit). For certain yeast and fungal species, an optional lyticase treatment can be used to improve yields.
The purified genomic DNA is of high quality and suitable for a wide range of downstream applications, including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis, and more.
Fungi/yeast genomic DNA isolation kit (Spin column)
This spin column-based kit allows for rapid purification of genomic DNA from yeast, fungi, and bacteria, including Gram-positive. A single sample can be processed in under 30 minutes, and each kit includes enough materials for 50 preparations.
Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)
Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable, and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores, or mycelium and Gram-positive bacteria. The purification can be carried out on either a vacuum manifold or via centrifugation. 96 purifications can be completed in just 40 minutes.
Details
Supporting data
Figure 1. DNA Isolation from Different Fungi Species and Yeast. To demonstrate the purification of DNA from different fungal species, 30 mg of fungi were collected from plate cultures of Pichia sp, Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea, and Mucor racemosus, and the DNA was extracted using Norgen's Fungi/Yeast Genomic DNA Isolation Kit. The bead system efficiently lysed the fungal cell walls with the provided Lysis Solution, and total DNA was eluted in 100 µL. For analysis, 10 µL from each elution was loaded in 1% 1xTAE agarose gel. Lane 1: Yeast (Pichia sp.), Lane 2: Aspergillus Niger; Lane 3: Cladosporium cladosporioides; Lane 4: Botrytis cinerea; Lane 5: Mucor racemosus; Lane M: Norgen's HighRanger 1 kb DNA Ladder. The optional RNase treatment was not performed during the process. Image Credit: Norgen Biotek Corporation
Figure 2. DNA can be Isolated from Spores and Mycelium. Genomic DNA was extracted from the spores and mycelium of Botrytis cinera using Norgen's Fungi/Yeast Genomic DNA Isolation Kit. For the spores, 105 spores/mL were collected from a plate culture by adding 3 mL of 0.9% NaCl to the fungi culture plate and gently shaking to extract the spores. For mycelium, 20 mg of mycelium was collected with sterilized forceps. DNA was isolated using Norgen's Fungi/Yeast Genomic DNA Isolation Kit with the provided Bead Tubes. For analysis, 10 µL from each 100 µL elution was loaded on a 1% 1xTAE agarose gel. Lane M: Norgen's HighRanger 1 kb DNA Ladder. Optional RNase treatment was not performed during the extraction. As it can be seen, DNA could be successfully isolated from both the spores and mycelium. Image Credit: Norgen Biotek Corporation
Figure 3. Identification of Fungal and Yeast Species in a Real-time PCR (SYBR Green). DNA was isolated from 50 mg samples (wet weight) of yeast (Pichia sp) and fungi including Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea, and Mucor racemosus using Norgen's Fungi/Yeast Genomic DNA Isolation Kit, and 2 µL of each of the 100 µL DNA elutions was used in a qPCR (SYBR Green) reaction with specific fungal and yeast primers. The qPCR was successful in amplifying and detecting all the yeast and fungal DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Image Credit: Norgen Biotek Corporation
Figure 4. High Yields of DNA Isolated from Saccharomyces cerevisiae overnight culture. DNA was isolated from S. cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Following isolation of 10 samples, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1 kb DNA Ladder. Image Credit: Norgen Biotek Corporation
Figure 5. High quality of yeast DNA measured by NanoDrop. DNA isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit showed a high quality of DNA based on A260/280 and A260/230. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. Image Credit: Norgen Biotek Corporation
Figure 6. Purified DNA Can be Amplified in a Real-time PCR (SYBER GREEN) Reaction. DNA was isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Five µL of the DNA from each 100 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with S. cerevisiae primers. The real-time PCR was successful in amplifying the S. cerevisiae from all 10 isolated samples. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control. Image Credit: Norgen Biotek Corporation
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Spin column
Source: Norgen Biotek Corporation
| Kit specifications |
| Column Binding Capacity |
50 μg |
| Maximum Column Loading Volume |
650 μL |
Maximum Amount of Starting Material:
Fungi (wet weight)
Yeast or Bacteria Culture |
50 mg
0.5 mL - 1 mL |
Average Yield*
Pichia sp. (yeast)
Botrytis cinerea
Fusarium sp.
Aspergillis niger
Mucor racemosus
Cladosporium cladosporioides
Penicillium sp.
Rhizopus oryzae
Alternaria tenuissima |
25 μg
32 μg
42 μg
26 μg
15 μg
12 μg
40 μg
7 μg
30 μg |
| Time to Complete 10 purifications |
30 minutes |
* Yield will vary depending on the type of sample processed
High throughput
Source: Norgen Biotek Corporation
| Kit specifications |
| Binding Capacity Per Well |
50 μg |
| Maximum Loading Volume Per Well |
500 μL |
| Size of DNA Purified |
All sizes |
Maximum Amount of Starting Material:
Fungi (Wet weight)
Yeast or Gram-positive bacterial culture |
50 mg
0.5 mL - 1 mL |
| Time to Complete 96 Purifications |
40 minutes |
Storage conditions and product stability
Every solution needs to be stored at room temperature and kept tightly sealed. In their unopened containers, these reagents should be stable for at least two years.
Source: Norgen Biotek Corporation
| Component |
Cat. 27300 (50 preps) |
Cat. 27350 (192 preps) |
| Lysis Buffer L |
30 mL |
2 x 60 mL |
| Resuspension Solution A |
20 mL |
60 mL |
| Solution BX |
28 mL |
2 x 28 mL |
| Wash Solution A |
18 mL |
2 x 38 mL |
| Elution Buffer B |
15 mL |
30 mL |
| Bead Tubes |
50 |
200 |
| Spin Columns |
50 |
- |
| Collection Tubes |
50 |
- |
| 96-Well Plate |
- |
2 |
| 96-Well Collection Plate |
- |
2 |
| Adhesive Tape |
- |
4 |
| Elution Tubes (1.7 mL) |
50 |
- |
| 96-Well Elution Plate |
- |
2 |
| Product Insert |
1 |
1 |