High-quality genomic DNA isolation from cells and animal tissues

Genomic DNA can be extracted from a variety of animal tissues or cell samples using Norgen's Cell and Tissue DNA Isolation Kits.

Format

Spin column

Size

50 preps

Image Credit: Norgen Biotek Corp.

Micro

Size

50 preps

Image Credit: Norgen Biotek Corp.

Magnetic bead system

Size

50 preps

Image Credit: Norgen Biotek Corp.

High throughput magnetic bead system

Size

2 × 96-Well Plates

Image Credit: Norgen Biotek Corp.

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Overview

Norgen's Cells and Tissue DNA Isolation Kits are intended for the fast extraction of genomic DNA from cultivated cells, tissue samples, and urine. The purified genomic DNA is fully digested by all restriction enzymes tested and is suitable for PCR and Southern Blot analysis.

Features and benefits

• DNA ready for any application, including PCR, qPCR, genotyping and more
• Fast, reproducible, and easy processing of samples
• High-yield and high-quality genomic DNA with no RNA or protein contamination

Cells and tissue DNA isolation kit (Spin column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low-salt, slightly alkaline conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and tissue DNA isolation micro kit (Micro)

This kit is optimized for small inputs of cells and tissues, including samples obtained through Laser-Captured Microdissection (LCM). DNA purification is based on spin column chromatography, using a separation matrix that binds DNA under optimized salt conditions and releases it under low-salt, slightly alkaline conditions. The protocol takes approximately 60 minutes per sample, and each kit includes enough materials for 50 preparations.

Cells and tissue DNA isolation kit (Magnetic bead system)

Purification is based on magnetic bead technology, where DNA binds under optimized conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) enables the extraction of genomic DNA from a wide range of animal tissues and cell samples. Each kit supports 50 purifications, with approximately 15 minutes of hands-on time using the automated method, or 40 minutes when using the manual protocol.

Cells and tissue DNA isolation 96-well kit (High throughput magnetic bead system)

Purification is based on magnetic bead technology, where DNA binds under optimized conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) enables the extraction of genomic DNA from a variety of animal tissues and cell samples.

The kit is also compatible with robotic automation systems for high-throughput workflows. Preparation time for 96 purifications is approximately 15 minutes of hands-on time with the automated method, or 60 minutes with the manual protocol.

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Details

Supporting data

Figure 1. Isolation of High Quality Genomic DNA from Tissue. Genomic DNA was isolated from 15 mg of liver tissue using Norgen's Cells and Tissue DNA Isolation Kit. Triplicate samples were used (lanes 1-3) and yielded genomic DNA of the highest quality and integrity. Lane M is the Norgen UltraRanger 1 kb DNA Ladder. For all purified DNA, 15 µL of each 200 µL elution were resolved on a 1X TAE, 1% agarose DNA gel. Image Credit: Norgen Biotek Corp.

Figure 2. Isolation of High Quality Genomic DNA from 0.1 up to 3 million mammalian cells. Genomic DNA was isolated from 0.1 x 10⁶ to up to 3 million HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Triplicate samples were used from each cell input and yielded genomic DNA of the highest quality and integrity with linear increase in yield with increasing cell input. Lane M is the Norgen UltraRanger 1 kb DNA Ladder. For all purified DNA, 15 µL of each 200 µL elution was resolved on a 1X TAE, 1% agarose DNA gel. Image Credit: Norgen Biotek Corp.

Figure 3. Yield of Genomic DNA from 0.1 x 10⁶ up to 3 x 10⁶ mammalian cells. Genomic DNA was isolated from 0.1 up to 3 million HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Triplicate samples were used from each cell input and 25 µL of the 200 µL elution were diluted in 475 µL of nuclease-free water, and DNA concentrations were measured using the UltraSpec 2100 Pro (Fisher Scientific). Linear increase in DNA yield was obtained when increasing the cell input from 0.1 × 10⁶ - 3 × 10⁶ HeLa cells. Image Credit: Norgen Biotek Corp.

Figure 4. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. Genomic DNA was isolated from 0.1 × 10⁶ to 3 × 10⁶ of HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Five µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene from all the isolated DNA from the different cell inputs with a decreasing Ct value with increasing the cell input. This indicates that the kit provides high-quality DNA in each case and is free of PCR inhibitors, making it well-suited to sensitive downstream applications. Image Credit: Norgen Biotek Corp.

Figure 5. Resolution of DNA isolated from Animal Tissue and Mammalian Cells. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System), and Norgen's Cells and Tissue DNA Kit (column format, Cat. 53100) as a control. The DNA isolated with the Magnetic Bead System (B) was then compared with the column method (C). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. As it can be seen, Norgen's Magnetic Bead System (B) was able to isolate high yields of DNA from both the tissue and cells. M = Norgen's HighRanger DNA Ladder. Image Credit: Norgen Biotek Corp.

Figure 6. Comparison of A260/280 and A260/230 ratios. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System), and Norgen's Cells and Tissue DNA Kit (column format, Cat. 53100). The DNA isolated with the Magnetic Bead System was then compared with the column method for A260/280 and A260/230 rations. DNA isolated using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System) showed a comparable DNA quality to the column method (Cat. 53100). Image Credit: Norgen Biotek Corp.

Figure 7. High Quality DNA was confirmed by Real-time PCR. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL (circle), 4 µL (triangle) and 8 µL cross) of animal tissue DNA or HeLa DNA (total PCR reaction volume was 20 µL) to detect 5s rDNA or GAPDH from animal tissue and HeLa cell respectably. No PCR inhibition was observed from the all different PCR template amounts, indicating the excellent quality of animal tissue and HeLa DNA for downstream applications. Image Credit: Norgen Biotek Corp.

Figure 8. DNA was Isolated from 1 x 10⁶ HeLa Cells Using Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from each 100 µL of elution were run on 1X TAE 1.2% agarose gel. As it can be seen, Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) was able to isolate consistent and high yields of DNA from HeLa cells. M = Norgen's HighRanger DNA Ladder (Cat. 11900). Image Credit: Norgen Biotek Corp.

Figure 9. High Quality DNA was Confirmed by Real-Time PCR. DNA was isolated from 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL of HeLa DNA (total PCR reaction volume was 20 µL) to detect GAPDH from HeLa cell. No PCR inhibition was observed indicating the excellent quality of HeLa DNA for downstream applications. Image Credit: Norgen Biotek Corp.

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Spin column

Source: Norgen Biotek Corp.

Storage Conditions and Product Stability: All solutions should be kept tightly sealed and stored at room temperature. The kit is stable for up to one year from the date of shipment. It includes a ready-to-use Proteinase K, dissolved in a specially formulated storage buffer. When stored at room temperature, the buffered Proteinase K remains stable for up to one year from the shipment date.

Micro

Source: Norgen Biotek Corp.

Storage Conditions and Product Stability: All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Magnetic bead system

Source: Norgen Biotek Corp.

* Average DNA yield will vary depending on the type of samples

Storage Conditions and Product Stability: All solutions must be stored at room temperature and kept tightly sealed. This kit is stable for one year following the date of shipment.

High throughput magnetic bead system

Source: Norgen Biotek Corp.

* Average DNA yield will vary depending on the type of samples

Storage Conditions and Product Stability: All solutions need to be kept tightly sealed and stored at room temperature. The kit is stable for one year following the date of shipment.

Source: Norgen Biotek Corp.