Total RNA and microRNA extraction from small input amounts

Norgen's Single Cell RNA separation Kit enables the fast separation of total RNA, including microRNA, from modest input amounts.

Designed to handle as few as a single cell and up to 100,000 cells, this kit is ideal for working with limited or precious samples. Its uniquely engineered micro spin-column allows for elution in volumes as low as 8 µL.

The streamlined 15-minute protocol efficiently isolates high-quality total RNA—including miRNA—from a variety of sample types, such as cultured animal cells, circulating tumor cells (CTCs), stem cells, immuno-sorted cells, and laser-capture microdissected (LCM) tissue. The purified RNA is of exceptional purity and integrity, making it ideal for downstream applications, including qRT-PCR, digital PCR, and whole transcriptome analysis.

Format

Spin column

Size

50 preps

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Features and benefits

• Small elution volume of 8 µL ready for direct utilization in qRT-PCR reactions
• Quick and easy processing using rapid micro spin column format
• Isolate high-quality total RNA from a variety of sources
• Isolate total RNA, including microRNA, in a concentrated ready-to-use format
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
• RNA can be isolated from a single cell to 100,000 cells

Details

Supporting data

Figure 1. Better Efficiency of RNA Recovery at Single Cell Input. Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor’s kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript in the isolated RNA. RNA isolated by Norgen’s Single Cell RNA Purification Kit showed much higher relative expression of each RNA transcript tested at all cell input numbers down to single cell. Image Credit: Norgen Biotek Corp.

Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM). Norgen’s Single Cell RNA Purification Kit allows sensitive but high-quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Single Cell RNA Purification Kit recovered good amounts of RNA with an excellent 260/280 ratio. Image Credit: Norgen Biotek Corp.

Figure 3. Consistent and Linear Recovery of All Sizes of RNA down to Single Cell Input. Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell with great linearity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit. The extracted RNA was subjected to RT-qPCR to detect all sizes of RNA, including the human S15 (mRNA), 5S rRNA transcript (small RNA), and miR-21 (microRNA). RNA isolated by Norgen’s Single Cell RNA Purification Kit showed very high consistency (over 99%) of recovery of each RNA transcript tested at all cell input numbers down to a single cell. Image Credit: Norgen Biotek Corp.

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Kit specifications

Source: Norgen Biotek Corp.

Storage conditions and product stability

All solutions need to be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Source: Norgen Biotek Corp.