High-Quality total RNA purification from lipid-rich animal tissues

The Fatty Tissue RNA Purification Kit from Norgen was designed to help purify total RNA (including microRNA) from lipid-rich animal tissues.

Overview

This kit was created to aid with the extraction of high-quality and yield RNA from fatty tissues such as the brain, breast, adipose tissues, etc. However, tissues with a high fat content, such as the brain and breast, can often lead to low-quality and yield RNA with a non-optimized kit.

This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA)—all without the need for inhibitory phenol or chloroform. Although originally intended for fatty tissues, the kit can also be used for most mammalian tissue samples.

The purified RNA is of the highest integrity and can be used in several downstream applications, including RT-qPCR, microarrays, and NGS.

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Format

Spin Column

Size

25 preps

Features and benefits

• Fast and easy processing using rapid spin-column format
• Genomic DNA removal without the use of nucleases
• Isolate high-quality total RNA from hard-to-extract fatty tissues
• Isolate total RNA, including microRNA (miRNA)
• No phenol or chloroform extractions
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Details

Supporting data

Figure 1. Isolation of High Quality Brain Total RNA including microRNA without the use of Phenol. Norgen's Fatty Tissue RNA Purification Kit isolates high quality total RNA from fatty tissues such as brain without the use of phenol. Total RNA was isolated from equal amounts of brain tissue using Norgen’s Fatty Tissue RNA Purification Kit and a leading competitor’s kit that involves a phenol:chloroform extraction step. The purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, Norgen not only isolated high and consistent yields of total RNA, but the RNA was also of high quality as evidenced by intactness of the major 28S and 18S rRNA. Importantly, only Norgen's Fatty Tissue RNA Purification Kit was able to recover the small RNA fraction, including the microRNA (red-boxed). Image Credit: Norgen Biotek Corp.

Figure 2. High Yield of a Diversity of RNA Species. Norgen's Fatty Tissue RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of brain tissue using Norgen's Fatty Tissue RNA Purification Kit and a leading competitor’s kit that involves a phenol:chloroform extraction step. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Top Panel) and for detecting miR-21 (Bottom Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Top Panel). Also, Norgen's kit successfully isolated similar amount of large RNA to that of the competitor's kit (Bottom Panel) indicating the full diversity of RNA species isolated. Image Credit: Norgen Biotek Corp.

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Kit specifications

Source: Norgen Biotek Corp.

*Average Yield will vary depending on a number of factors including species, growth conditions used, and developmental stage.

Storage conditions and product stability

All solutions must be stored at room temperature and should remain tightly sealed. This kit will be stable for two years from the date of dispatch.

Source: Norgen Biotek Corp.