Norgen's Genomic DNA Isolation Kit isolates genomic DNA from animal tissues, cells, bodily fluids, viruses, and swabs.
Overview
This kit was created to aid in the the speedy preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol.
The purified DNA produced via this method has an excellent yield and quality and is ready for any immediate downstream application, including PCR, qPCR, genotyping, sequencing, and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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Format
Spin column
Size
50 preps
100 preps
250 preps
Features and benefits
- A rapid and convenient spin-column procedure
- Isolate genomic DNA from animal tissues, cells, bodily fluids, viruses and swabs
- The purified DNA is of the highest quality and integrity for sensitive downstream applications, including PCR, qPCR, genotyping, sequencing, and more
Details
Supporting data
Figure 1. Isolation of High Quality Genomic DNA. Genomic DNA was isolated from various sample types using Norgen's Genomic DNA Isolation Kit. Lanes 1-3 contain genomic DNA that was isolated from four different samples containing 5 x 105 HeLa cells, while lanes 4 and 5 contain genomic DNA that was isolated from 5 mg of heart tissue. All the purified genomic is of the highest quality and integrity. Lane M is the Norgen HighRanger 1 kb DNA Ladder. For all purified DNA, 10 µL of each 200 µL elution were resolved on a 1X TAE, 1% agarose DNA gel. Image Credit: Norgen Biotek Corp.
Figure 2. Full Compatibility with Digests. Genomic DNA was isolated from 10 mg of kidney tissue using Norgen's Genomic DNA Isolation Kit, and subsequently digested. Twenty microliters of the 200 µL eluted DNA were used in a 50 µL restriction digestion with 10 units of either Hind III or Nde I (overnight at 37 °C). Fifteen microliters of the digested DNA were then loaded on a 1X TAE, 1% agarose gel. Lane 1 contains undigested Norgen-isolated DNA, while Lanes 2 contain the genomic DNA digested with Hind III and Lanes 3 contain the genomic DNA digested with Nde I. Lane M is Norgen's HighRanger 1 kb DNA Ladder. Image Credit: Norgen Biotek Corp.
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Kit specifications
Source: Norgen Biotek Corp.
| Kit Specifications |
| Column Binding Capacity |
25 µg |
Average Yield:*
HeLa Cells (1 x 106)
Tissue (from 10 mg kidney) |
8 µg
10 µg |
Maximum Amount of Starting Material:
Animal Tissues
Cultured Cells
Bodily Fluids (blood, saliva)
Viral Suspension |
20 mg
3 x 106 cells
150 µL
150 µL |
| Time to Complete 10 Purifications |
80 minutes |
* Yield will vary depending on the type of sample processed
Storage conditions and product stability
Both upon arrival and following reconstitution, the Proteinase K should be kept at –20 °C. Store all other solutions at room temperature in firmly sealed containers. This kit is stable for two years after the date of shipment.
Source: Norgen Biotek Corp.
| Component |
Cat. 24700 (50 preps) |
Cat. 24750 (100 preps) |
Cat. 24770 (250 preps) |
| Digestion Buffer A |
25 mL |
2 x 25 mL |
5 x 25 mL |
| Buffer SK |
30 mL |
2 x 30 mL |
5 x 30 mL |
| Wash Solution A |
18 mL |
2 x 18 mL |
5 x 18 mL |
| Elution Buffer B |
30 mL |
2 x 30 mL |
5 x 30 mL |
| Proteinase K |
12 mg |
2 x 12 mg |
5 x 12 mg |
| Spin Columns |
50 |
100 |
250 |
| Collection Tubes |
50 |
100 |
250 |
| Elution Tubes (1.7 mL) |
50 |
100 |
250 |
| Product Insert |
1 |
1 |
1 |