Rapid and efficient bacterial genomic DNA isolation

The Bacterial Genomic DNA Isolation Kit from Norgen allows users to prepare bacterial genomic DNA quickly.

Format

Spin column

Size

50 preps

Image Credit: Norgen Biotek Corp.

Image Credit: Norgen Biotek Corp.

High throughput

Size

2 × 96-Well Plates

Image Credit: Norgen Biotek Corp.

Download the Brochure for More Information

Overview

This kit was created to enable the rapid spin column-based extraction of genomic DNA from up to 2 × 10⁹ viable bacterial cells (typically from 0.5 to 1.0 mL of culture). It’s suitable for both Gram-negative and Gram-positive bacteria, including Escherichia coli and Bacillus cereus.

The purified genomic DNA is high in both quality and yield, making it fully compatible with a wide range of downstream applications such as restriction enzyme digestion, sequencing, PCR, qPCR, and more. The kit is also available in a convenient 96-well format for high-throughput workflows.

Features and benefits

• 96-well format available for high-throughput
• High yield, high-quality DNA for sensitive downstream applications, including sequencing, PCR, qPC,R and more
• Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
• Rapid and convenient spin column protocol

Details

Supporting data

Figure 1. Isolation of Genomic DNA from both Gram Positive and Gram Negative Bacteria. The Bacterial Genomic DNA Isolation Kit was used to isolate genomic DNA from 1 mL overnight culture (1 x 10⁹ cells) of the Gram negative bacteria E. coli (Lanes 1 and 2), the lysozyme-resistant Gram positive bacteria B. cereus (Lanes 3 and 4) and the Gram positive bacteria M. luteus (Lanes 5 and 6). Lane M is Norgen's UltraRanger 1 kb DNA Ladder. For analysis 5 µL of the 200 µL eluted genomic DNA were loaded on a 1X TAE, 0.9% agarose gel. Image Credit: Norgen Biotek Corp.

Figure 2. High Yield Purification. The high yield of Norgen's Bacterial Genomic DNA Isolation Kit is illustrated by purifying genomic DNA from 1 mL overnight culture (1 x 10⁹ cells) of both a Gram positive (B. cereus) and a Gram negative strain (E coli), and comparing the yield with two major competitors. The quantification of the DNA yield was performed by resolving 5 µL of the 200 µL of eluted DNA on a 1X TAE, 0.9% agarose gel followed by densitometry. With both types of bacteria, Norgen's kit was found to give a higher recovery than the competitor’s kits. Image Credit: Norgen Biotek Corp.

Download the Brochure for More Information

Spin column

Source: Norgen Biotek Corp.

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability: All solutions must be stored at room temperature and kept tightly sealed. This kit is stable for two years from the dispatch date. It contains ready-to-use Proteinase K dissolved in a specially prepared storage buffer. When stored at room temperature, the buffered Proteinase K is stable for up to two years after the date of shipment.

High throughput

Source: Norgen Biotek Corp.

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability: All solutions need to be stored at room temperature and kept tightly sealed. This kit is stable for one year following the dispatch date. It contains ready-to-use Proteinase K dissolved in a specially prepared storage buffer. When stored at room temperature, the buffered Proteinase K is stable for up to one year after the date of shipment.

Source: Norgen Biotek Corp.