Interleukin-2 (IL-2) receptors from ACROBiosystems

Interleukin-2 (IL-2) is a pluripotent cytokine that regulates the immune system’s response. The IL-2 receptor (IL-2R) is a heterotrimeric protein that binds to IL-2 and reacts to it on the surface of some immune cells, like lymphocytes.

IL-2R is made up of three subunits: IL-2Rα (CD25), IL-2Rβ (CD122) and common γc (CD132). The three receptor chains can assemble in multiple configurations and sequences to form low, moderate, and high-affinity IL-2 receptors, and they are expressed individually and variably on distinct cell types.

PEG modification, IL-2 mutant design, fusion Fc, bispecific antibody design, combination immunization checkpoint antibody medication treatment, and other tactics have all been used by biopharma and biotech companies to modify and develop IL-2.

In vitro investigations of the interaction between IL-2 and IL-2 receptors, as well as antibody immunization and screening, need a series of structurally stable and high-affinity IL-2 receptor heterodimers and heterotrimers.

ACROBiosystems has created a series of IL-2-related proteins with high purity, stability, and bioactivity, including IL-2, IL-2Rα (CD25), IL-2Rβ (CD122), IL-2Rγ (CD132), IL-2 R βγ heterodimer, IL-2Rαβγ heterotrimer, which can be utilized to study the interaction between IL-2 and IL-2 receptors, antibody screening, immunization, and other applications to help in creating IL-2-related therapies.

Figure 1. Native IL-2 has pleiotropic effects on the immune response. Image Credit: ACROBiosystems

Figure 2. Schematic of IL-2 binding to the low-affinity, intermediate-affinity and high-affinity IL-2 receptors. The high-affinity receptor comprises IL-2 receptor α-chain (IL-2Rα), IL-2Rβ and IL-2Rγ. Image Credit: ACROBiosystems

Features

• Affinity verified by SPR and BLI with free protocols
• Various tags: Fc, His, Fc and Avi, His and Strep, Tag Free
• High stability: strict quality control to ensure high batch-to-batch consistency
• Various species: Human, mouse, cynomolgus, rat, canine
• High purity: SDS-PAGE verification purity >95%, SEC-MALS verification purity >90%
• High bioactivity verified by ELISA with free protocols

Product list

IL-2

Table 1. Source: ACROBiosystems

Molecule	Cat.No.	Host	Product description	Structure
IL-2	IL2-H4113	E.coli	Human IL-2 Protein, Tag Free
	IL2-H5269	HEK293	Human IL-2 Protein, Fc Tag (MALS verified)
	IL2-H52H8	HEK293	Human IL-2 Protein, His Tag
	IL2-H82E4	HEK293	Biotinylated Human IL-2 Protein, His,Avitag™
	IL2-H82F3	HEK293	Biotinylated Human IL-2 Protein, Fc,Avitag™
	IL2-M52H3	HEK293	Mouse IL-2 Protein, His Tag

IL-2Rα

Table 2. Source: ACROBiosystems

Molecule	Cat.No.	Host	Product description	Structure
IL-2 R alpha	ILA-H5251	HEK293	Human IL-2 R alpha / CD25 Protein, Fc Tag (HPLC-verified)
	ILA-H52H9	HEK293	Human IL-2 R alpha / CD25 Protein, His Tag
	ILA-H82E6	HEK293	Biotinylated Human IL-2 R alpha / CD25 Protein, His,Avitag™
	ILA-H82F9	HEK293	Biotinylated Human IL-2 R alpha / CD25 Protein, Fc,Avitag™
	ILA-M52H9	HEK293	Mouse IL-2 R alpha / CD25 Protein, His Tag
	ILA-C52H4	HEK293	Canine IL-2 R alpha / CD25 Protein, His Tag

IL-2Rβ

Table 3. Source: ACROBiosystems

Molecule	Cat.No.	Host	Product description	Structure
IL-2 R beta	CD2-H5221	HEK293	Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)
	ILB-H5253	HEK293	Human IL-2 R beta / CD122 Protein, Fc Tag (MALS & SPR verified)
	ILB-H82E3	HEK293	Biotinylated Human IL-2 R beta / CD122 Protein, His,Avitag™
	ILA-M52H5	HEK293	Mouse IL-2 R beta Protein, His Tag
	ILB-M5253	HEK293	Mouse IL-2 R beta / CD122 Protein, Fc Tag
	ILA-C52H5	HEK293	Canine IL-2 R beta Protein, His Tag

IL-2Rγ

Table 4. Source: ACROBiosystems

Molecule	Cat.No.	Host	Product description	Structure
IL-2 R gamma	ILA-H52H5	HEK293	Human IL-2 R gamma / CD132 Protein, His Tag (MALS verified)
	ILG-H5256	HEK293	Human IL-2 R gamma / CD132 Protein, Fc Tag
	ILG-H85E8	InsectCells	Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™ (SPR verified)
	ILA-M52H3	HEK293	Mouse IL-2 R gamma / CD132 Protein, His Tag (MALS verified)

IL-2Rβγ

Table 5. Source: ACROBiosystems

Molecule	Cat.No.	Host	Product description	Structure
IL-2 R beta & IL-2 R gamma	ILG-H5254	HEK293	Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
	ILG-H5283	HEK293	Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)
	ILG-H82F3	HEK293	Biotinylated Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)
	ILG-M5254	HEK293	Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
	ILG-M5283	HEK293	Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)
	ILG-R5283	HEK293	Rat IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)
	ILG-C82F4	HEK293	Biotinylated Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

IL-2Rαβγ

Table 6. Source: ACROBiosystems

More IL proteins

• IL-1 beta
• IL-4
• IL-5
• IL-6
• IL-7
• IL-8
• IL-10
• IL-11
• IL-12B & IL-12A
• IL-13
• IL-15
• IL-17
• IL-21
• IL23A & IL12B
• IL-33

Assay data

Affinity verified by SPR

The SPR results of IL-2 Binding to IL-2 receptors coincide with the reference

Table 7. Affinity of IL-2 binding to IL-2R (SPR). Source: ACROBiosystems

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 40.6 pM as determined in an SPR assay (Biacore 8K). Image Credit: ACROBiosystems

Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILG-H5254) captured on CM5 chip via anti-human IgG Fc antibody can bind IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.123 nM as determined in an SPR assay (Biacore T200). Image Credit: ACROBiosystems

Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 29.9 nM as determined in an SPR assay (Biacore T200). Image Credit: ACROBiosystems

Human IL-2 R beta, His Tag (SPR verified) (Cat. No. CD2-H5221) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.525 μM as determined in a SPR assay (Biacore T200). Image Credit: ACROBiosystems

Biotinylated Human IL-2 R gamma, His, Avitag (Cat. No. ILG-H85E8) immobilized on SA Chip can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 105 μM as determined in a SPR assay (Biacore T200). Image Credit: ACROBiosystems

High purity heteropolymer verified by MALS

The purity of Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)(Cat. No. ILG-H5254) is more than 90% and the molecular weight of this protein is around 145-165 coda verified by SEC-MALS. Image Credit: ACROBiosystems

The purity of Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag(Cat. No. ILG-H5257) is more than 90% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS. Image Credit: ACROBiosystems

High bioactivity verified by ELISA

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4113) at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) with a linear range of 5-78 ng/mL. Image Credit: ACROBiosystems

Immobilized Biotinylated Human IL-2, His, Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) with a linear range of 0.4-6 ng/mL. Image Credit: ACROBiosystems