ACROBiosystems' GMP-grade human VCAM-1 proteins are produced and quality control tested to ensure GMP compliance.
Features
- Designed in compliance with ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested in a GMP-compliant facility
- Produced using animal-free materials
- Free from beta-lactam materials
- Ensuring batch-to-batch consistency
- Subjected to rigorous quality control testing
Source
GMP Human VCAM-1 Protein, Fc Tag (GMP-VC1H25) is expressed in human 293 cells (HEK293) and comprises amino acids Phe25–Glu698 (Accession #P19320-1).
Predicted N-terminus: Phe 25
Molecular characterization
Image Credit: ACROBiosystems Inc
This protein features a human IgG1 Fc tag at the C-terminus.
Due to glycosylation, it has a calculated MW of 100.7 kDa but migrates at 126 kDa ± 5 kDa under reducing (R) conditions in SDS-PAGE, as calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
<10 EU/mg by the LAL method.
Protein A
ELISA test reveals <5 ppm of protein.
Host cell protein
ELISA test shows <0.5 ng/µg of protein.
Host Cell DNA
qPCR quantification shows <0.02 ng/μg of protein.
Sterility
Sterility testing was conducted using the membrane filtration method as outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from a 0.22 μm filtered PBS solution, pH 7.4, with protectants.
Shipping
This product is supplied and shipped with blue ice.
Storage
Store immediately at -20 °C or lower for long-term storage.
Avoid repeated freeze-thaw cycles.
Stability under storage conditions:
- -70 °C for 12 months under sterile conditions after reconstitution
- -20 °C to -70 °C for 5 years in lyophilized state
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc
GMP Human VCAM-1 Protein, Fc Tag was analyzed by SDS-PAGE under reducing (R) conditions and stained with Coomassie Blue, confirming a purity greater than 95% (with Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Image Credit: ACROBiosystems Inc
Immobilized GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) at 5 μg/mL (100 μL/well) binds Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag & Tag Free (Cat. No. IT1-H82W1) with a linear range of 2–63 ng/mL (routinely tested).
Bioactivity-SPR
Image Credit: ACROBiosystems Inc
Captured GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) on a Protein A Chip binds Human ITGA4&ITGB1 Heterodimer Protein, His Tag & Tag Free (Cat. No. IT1-H52W1) with an affinity constant of 1.00 nM – 50.0 nM, as determined by SPR assay (Biacore 8K) (QC tested).
Application data
Image Credit: ACROBiosystems Inc
The premium-grade Recombinant Fibronectin fragment (Cat. No. FIN-H5116), in combination with GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coating on a plate effectively induces hematopoietic stem cell differentiation into NK cells with high CD56+ CD3—expression.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc
The cell-based assay reveals that GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) remains stable at 37 ℃ for 24 hours.
Image Credit: ACROBiosystems Inc
The SPR based assay reveals that GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) is stable at 4 ℃ for 180 days.
Image Credit: ACROBiosystems Inc
The cell-based assay shows that GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25), remains stable after freezing and thawing three times.
Image Credit: ACROBiosystems Inc
The SPR-based demonstrates batch-to-batch consistency between Acro's GMP and PG VCAM-1.