Transforming growth factor beta 1 (TGFB1), also known as TGF-β1, CED, DPD1, and TGFB, is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein with diverse cellular functions, including regulating cell growth, proliferation, differentiation, motility, and apoptosis.
TGFB1 is present throughout the body and is crucial in prenatal development, blood vessel formation, muscle tissue regulation, fat development, wound healing, and immune system function. It is particularly abundant in skeletal tissues, where it helps regulate bone growth, and in the extracellular matrix, which provides structural support between cells. Within cells, TGFB1 remains inactive until it receives a chemical signal to become active.
TGFB1 plays a key regulatory role in the immune system, exhibiting different effects depending on the cell type or developmental stage. Most immune cells (leukocytes) secrete TGFB1, influencing immune responses and maintaining tissue homeostasis. TGFB1 also interacts with several proteins, including TGF beta receptor 1, LTBP1, YWHAE, EIF3I, and Decorin, further contributing to its broad biological functions.
Features
- Developed in accordance with ISO 9001:2015 and ISO 13485:2016
- Manufactured and quality control tested in a GMP-compliant facility
- Free from animal-derived materials
- Free from beta-lactam materials
- Batch-to-batch consistency
- Subjected to rigorous quality control tests
Source
GMP Human TGF-Beta 1 Protein (GMP-TG1H25) is expressed in human 293 cells (HEK293) and consists of amino acids Ala 279 - Ser 390 (Accession # P01137-1).
Predicted N-terminus: Ala 279
Molecular characterization
Image Credit: ACROBiosystems Inc
This protein does not contain a “tag.”
Its calculated molecular weight is 12.8 kDa, and it migrates as 13 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE, calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
Endotoxin levels less than 10 EU/mg by the LAL method
Host cell protein
<0.5 ng/µg of protein tested by ELISA
Host cell DNA
qPCR reveals <0.02 ng/μg of protein
Sterility
Sterility testing was conducted using the membrane filtration method specified in CP<1101>, USP<71>, and Eur. Ph. 2.6.1
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE
Formulation
The protein is lyophilized from a 0.22 μm filtered solution in 100 mM HAC, pH 3.0, with protectants.
Shipping
The product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or below for long-term storage.
Avoid multiple freeze-thaw cycles.
The product maintains stability after storage at:
- -70 °C for 12 months under sterile conditions after reconstitution
- -20 °C to -70 °C for 5 years in lyophilized state
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc
With Coomassie Blue staining, GMP Human TGF-Beta 1 Protein is analyzed on SDS-PAGE under both reducing (R) and non-reducing (NR) conditions. The protein's purity exceeds 95%, as shown with the Star Ribbon Pre-stained Protein Marker.
Bioactivity-CELL BASE
Image Credit: ACROBiosystems Inc
GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) inhibits the GMP Human IL-4 Protein (Cat. No. GMP-L04H26) dependent proliferation of TF-1 cells. The specific activity of GMP Human TGF-Beta 1 Protein is > 6.00 × 10⁶ IU/mg, calibrated against transforming growth factor β1 (NIBSC code: 89/514) (QC tested).
Image Credit: ACROBiosystems Inc
The activity of GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) is higher compared to other competing products.
Application data
Image Credit: ACROBiosystems Inc
GMP Human FGF basic Protein (Cat. No. GMP-FGCH17) and GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) work together with GMP Human Laminin 521 Protein (Cat. No. GMP-LA5H24) to maintain the stemness of hiPSCs, promoting high expression of stem cell markers OCT4, SOX2, and SSEA4.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc
Cell-based assays demonstrate that GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) remains stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc
The cell-based assay reveals that GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) is stable at 4 °C for 30 days.
Image Credit: ACROBiosystems Inc
The cell-based assay demonstrates that GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) is stable after freezing and thawing three times.
Image Credit: ACROBiosystems Inc
There is consistent batch-to-batch performance between ACRO's GMP and PG TGF-Beta 1, as verified by cell-based assays.