Thrombopoietin (TPO) is a 332-amino-acid glycoprotein primarily produced in the liver and is the key physiological regulator of platelet production. It binds to the TPO receptor, activating the JAK-STAT signaling pathways to stimulate megakaryocyte growth and platelet formation. Research has also shown that TPO plays a crucial role in hematopoietic recovery following myeloablation, with its transcripts expressed by multiple cell types.
Features
- Manufactured and QC tested in a GMP-compliant facility
- Designed according to ISO 9001:2015 and ISO 13485:2016 standards
- Free from beta-lactam materials
- Made with animal-free materials
- Consistent quality across batches
- Subjected to stringent quality control testing
Source
GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) is expressed in human 293 cells (HEK293) and consists of amino acids Ser 22 to Gly 353 (Accession # P40225-1).
Predicted N-terminus: Ser 22
Molecular characterization
Image Credit: ACROBiosystems Inc
This protein is produced without any tag.
It has a calculated molecular weight (MW) of 35.5 kDa and migrates at approximately 75 kDa ± 5 kDa under reducing (R) conditions in SDS-PAGE due to glycosylation when calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
Endotoxin level is less than 10 EU/mg, as determined by the LAL method.
Host cell protein
<0.5 ng/µg of protein tested by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Sterility is tested using the membrane filtration method according to CP<1101>, USP<71>, and Eur. Ph. 2.6.1
Mycoplasma
Negative.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from a 0.22 μm filtered solution in 20 mM NaAc-HAc buffer (pH 5.0) with protectants.
Shipping
GMP Human Thrombopoietin Protein is supplied and shipped with blue ice.
Storage
Store immediately at -20 °C or lower upon receipt for long-term storage.
Avoid repeated freeze-thaw cycles.
This product remains stable under the following conditions:
- -20 °C to -70 °C for five years in lyophilized state
- -70 °C for 12 months under sterile conditions after reconstitution.
ACRO quality management system
- Quality Control Process
- QMS（ISO, GMP）
- Quality Advantages
QC and assay protocol
SDS-PAGE
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SDS-PAGE analyzed GMP Human Thrombopoietin Protein under both reducing (R) and non-reducing (NR) conditions, with the gel stained using Coomassie Blue. The purity of the protein is greater than 95%, as determined with the Star Ribbon Pre-stained Protein Marker.
Bioactivity-Bioactivity CELL BASE
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GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) stimulates the proliferation of Mo7e cells. The specific activity of GMP Human Thrombopoietin Protein is greater than 1.00 × 107 IU/mg, calibrated against the human TPO Standard (NIBSC code: 03/124) (QC tested).
Image Credit: ACROBiosystems Inc
The activity of GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) exceeds that of competing products.
Application data
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GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17), and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) significantly promote the differentiation of iPSCs into HSPCs after 14 days, with high expression of hematopoietic stem cell markers CD34 and CD45.
Image Credit: ACROBiosystems Inc
GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) is essential for the expansion of human CD34+ hematopoietic cells when cultured in a medium containing GMP Human SCF Protein (Cat. No. GMP-SCFH25) and GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28). Cell viability was assessed using a luminescent cell viability detection reagent.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc
The cell-based assay demonstrates that GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) remains stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc
The cell-based assay demonstrates that GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) remains stable at 4 °C for 180 days.
Image Credit: ACROBiosystems Inc
The cell-based assay further indicates that GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25) maintains stability after three freeze-thaw cycles.
Image Credit: ACROBiosystems Inc
The cell-based assay shows batch-to-batch consistency between Acro's GMP and PG Thrombopoietin.