Platelet-derived growth factors (PDGFs) are mitogenic during early development, promoting the proliferation of undifferentiated mesenchymal cells and specific progenitor populations. At later maturation stages, PDGF signaling plays a role in tissue remodeling, cellular differentiation, and key inductive events involved in patterning and morphogenesis. Beyond stimulating mesenchymal proliferation, PDGFs also regulate the migration, differentiation, and function of various specialized mesenchymal and migratory cell types during development and adulthood.
Other members of this growth factor family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C), which are involved in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF), which also contributes to angiogenesis.
PDGF is essential for embryonic development, cell proliferation, migration, and angiogenesis. It plays a critical role in fibroblast division, a key process in connective tissue maintenance, and supports the proliferation of oligodendrocyte progenitor cells.
Platelet-derived growth factor subunit B (PDGFB), also known as FLJ12858, PDGF2, SIS, SSV, and c-sis, belongs to the PDGF family. It can form either a homodimer (PDGF-BB) or a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), with the dimers linked by disulfide bonds. Mutations in PDGFB have been associated with meningioma.
Features
- Developed in accordance with ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Utilizes materials that are free from animal sources
- Ensures consistency across batches
- Free from beta-lactam materials
- Subjected to rigorous quality control testing
- Does not incorporate animal-derived peptone or lactose in the manufacturing process
Source
GMP Human PDGF-BB Protein (GMP-PDBH19) is produced in E. coli cells and consists of amino acids Ser 82 to Thr 190 (Accession # P01127-1).
Predicted N-terminus: Met
Molecular characterization
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This protein does not have a "tag."
It has a calculated MW of 12.4 kDa and migrates as 13 kDa ± 3 kDa under reducing conditions (SDS-PAGE).
Endotoxin
Less than 10 EU/mg as measured by the LAL method.
Host cell protein
<0.5 ng/µg of protein determined by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Sterility testing was conducted using the membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1. T
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid - Sodium Citrate, pH 3.0 with protectants.
Shipping
The product is supplied and shipped with blue ice.
Storage
Upon arrival, immediately store at -20 °C or lower for long-term storage.
Avoid multiple freeze-thaw cycles.
This product remains stable when stored at:
- -20 °C to -70 °C for 5 years in lyophilized form
- -70 °C for 12 months under sterile conditions after reconstitution.
ACRO quality management system
- Quality Advantages
- QMS（ISO, GMP
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc
SDS-PAGE analysis of GMP Human PDGF-BB Protein in both reducing (R) and non-reducing (NR) conditions. Coomassie Blue was used to stain the gel. The protein has a purity of over 95%.
Bioactivity-Bioactivity CELL BASE
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GMP Human PDGF-BB Protein (Cat. No. GMP-PDBH19) promotes NIH/3T3 cell growth. The human PDGF-BB WHO International Standard (NIBSC code: 94/728) is used to calibrate the specific activity of GMP Human PDGF-BB Protein, which is >5.00 x 105 IU/mg (QC tested).
Image Credit: ACROBiosystems Inc
The activity of GMP Human PDGF-BB Protein (Cat. No. GMP-PDBH19) exceeds that of other competing products.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc
Cell-based assays demonstrate that GMP Human PDGF-BB Protein (Cat. No. GMP-PDBH19) remains stable at 4 °C for 180 days.
Image Credit: ACROBiosystems Inc
Cell-based assays also show that GMP Human PDGF-BB Protein (Cat. No. GMP-PDBH19) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc
The cell-based assay reveals that GMP Human PDGF-BB Protein (Cat. No. GMP-PDBH19) is stable after freezing and thawing three times.