Laminin 511 E8 (LN511-E8) is a recombinant human protein that offers a defined surface for feeder-free culture of various human pluripotent stem cells (PSCs) in vitro. As a truncated version of laminin 511, LN511-E8 is a functionally minimal form that retains the full ability to bind to integrins.
Without inducing differentiation, LN511-E8 has been shown to sustain normal growth characteristics and preserve stemness in multiple PSC lines, including ESC, iPSC, and MSC. Furthermore, LN511-E8 has demonstrated the ability to support PSC growth for over 10 passages without any signs of karyotypic abnormalities while maintaining the PSCs' capacity to differentiate into all three germ layers.
As Takamichi Miyazaki et al. reported, the LN511-E8 variant of laminin 511 demonstrates higher efficiency in supporting the adhesion of dissociated cells compared to wild-type laminin 511, making it a more cost-effective choice.
Optimized and defined surface
Laminin 511 E8 (LN511-E8) has been shown to support single-cell seeding at low density. The seeded cells exhibit high motility and improved clonal survival in a feeder-free culture system.
Physiology-related
The laminin 511 isoform plays a crucial role in the growth and maintenance of human pluripotent stem cells (hPSCs) through its interaction with the a6B1 integrin receptors. LN511-E8, a truncated isoform of laminin 511, is effective across various stem cell lines, including iPSC, hESC, and MSC.
Better adhesion
The LN511-E8 variant of laminin 511 is more efficient than wild-type laminin 511 in supporting the adhesion of dissociated cells.
Cost-effective
LN511-E8 maintains pluripotency at a lower dose without inducing differentiation, making it a more cost-effective option than laminin 521 for the feeder-free culture of human pluripotent stem cells (PSCs).
Reduce variability
As a defined recombinant human protein, LN511-E8 offers improved lot-to-lot consistency, reducing variability in PSC cultures.
Features
- Developed following ISO 9001:2015 and ISO 13485:2016 standards
- Produced and quality-controlled in a GMP-compliant facility
- Contains no beta-lactam materials
- Composed of animal-free materials
- Consistent quality across batches
- Undergoes rigorous quality control testing
Key parameter
Source: ACROBiosystems Inc
| . |
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| Mycoplasma Test |
Negative |
| Sterility Test |
Negative |
Formulation
ACRO offers detailed product information upon request.
Shipping
The product is delivered and shipped with dry ice.
Storage
For long-term storage, the product must be kept in its liquid form at -70 °C.
Avoid repeated freeze-thaw cycles.
The product is stable when stored at:
- 2-8 °C for up to six months under sterile conditions
- -20 °C or lower for up to 3 years
ACRO quality management system
- Quality Control Process
- QMS（ISO, GMP）
- Quality Advantages