Tumor necrosis factor ligand superfamily member 9 (4-1BBL), also known as 4-1BB ligand, CD137L, or TNFSF9, is a cytokine that binds to TNFRSF9. It serves as the high-affinity ligand for 4-1BB and plays a key role in promoting the proliferation of activated peripheral blood T cells.
Additionally, 4-1BBL may contribute to activation-induced cell death (AICD) and facilitate interactions between T cells and B cells or macrophages. It is also implicated in various diseases, including cancers, infectious diseases, and autoimmune disorders.
Introduction
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Animal-free materials
- Beta-lactam-free formulation
- Consistent batch-to-batch quality
- Undergoes stringent quality control testing
Source
GMP Human 4-1BB Ligand Protein, Fc Tag (GMP-41LH26) is expressed in HEK293 human cells. It includes amino acids Arg 71 - Glu 254 (Accession # P41273-1).
Predicted N-terminus: Pro
Molecular characterization
Image Credit: ACROBiosystems
This protein features a human IgG1 Fc tag at the N-terminus.
It has a calculated molecular weight (MW) of 87.5 kDa. Due to glycosylation, it migrates at 95 kDa ± 5 kDa under reducing (R) conditions in SDS-PAGE, as calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
Using the LAL method, less than 10 EU/mg.
Protein A
ELISA-tested protein at less than 5 ppm.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative.
Purity
>95 %, according to SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term storage.
Avoid repeated freeze-thaw cycles.
This product remains stable under the following recommended storage conditions:
- In a lyophilized state, -20 °C to -70 °C for 5 years
- After reconstitution, -70 °C for 12 months under sterile conditions
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Human 4-1BB Ligand Protein, Fc Tag was analyzed by SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue, and the protein's purity exceeds 95 %, as confirmed using the Star Ribbon Pre-stained Protein Marker.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems
GMP Human 4-1BB Ligand Protein, Fc Tag (Cat. No. GMP-41LH26) induces IL-8 secretion in the HT1080 human CD137 cell line. The specific activity of GMP Human 4-1BB Ligand Protein, Fc Tag is >1.00 × 105 U/mg (QC tested).
Image Credit: ACROBiosystems
Compared to competing products, GMP Human 4-1BB Ligand Protein, Fc Tag (Cat. No. GMP-41LH26) exhibited a higher level of activity.
Bioactivity-Stability
Image Credit: ACROBiosystems
GMP Human 4-1BB Ligand Protein, Fc Tag (Cat. No. GMP-41LH26) is stable at 37 °C for 16 hours, according to the cell-based assay.
Image Credit: ACROBiosystems
GMP Human 4-1BB Ligand Protein, Fc Tag (Cat. No. GMP-41LH26) is stable at 4 °C for 180 days, according to the cell-based assay.
Image Credit: ACROBiosystems
GMP Human 4-1BB Ligand Protein, Fc Tag (Cat. No. GMP-41LH26) is stable after three freezing and thawing cycles, according to the cell-based assay.
Image Credit: ACROBiosystems
Batch-to-batch consistency between ACRO's GMP and PG 4-1BB Ligand is demonstrated by the cell-based assay.