The T-cell surface glycoprotein CD5, also known as Lymphocyte antigen T1/Leu-1 and LEU1, is phosphorylated on tyrosine residues by LYN, allowing CD5 to create binding sites for PTPN6/SHP-1. CD5 may function as a receptor that regulates T-cell proliferation. It is expressed at various stages of development and activation on human B cells.
CD5 is a well-established negative regulator of TCR and BCR signaling. CD5-positive cells may help prevent the development of autoimmunity by producing cytokines such as IL-10. The development, selection, and function of various B- and T-cell subsets, or their preferential survival, may depend directly or indirectly on the glycan structures associated with CD5 or CD5-like molecules.
Features
- Designed according to ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and quality control tested in a GMP-compliant facility
- Made with animal-free materials
- Free from beta-lactam materials
- Consistent batch-to-batch quality
- Subjected to stringent quality control testing
Source
GMP Biotinylated Human CD5 Protein, His, Avitag (GMP-CD5H24) is expressed in human 293 cells (HEK293). It consists of amino acids Arg 25 to Pro 372 (Accession # P06127-1).
N-terminus prediction: Arg 25
Molecular characterization
Image Credit: ACROBiosystems
This protein contains a polyhistidine tag at the C-terminus, followed by an Avi tag (AvitagTM).
The protein has a calculated molecular weight of 42.2 kDa. Under reducing conditions (SDS-PAGE), the protein migrates at approximately 52 kDa ± 3 kDa when calibrated against the Star Ribbon Pre-stained Protein Marker due to glycosylation.
Labeling
AvitagTM technology is used to biotinylate this product. In short, biotin is enzymatically labeled to the Avitag's single lysine residue.
Biotinylation
As determined by Quantitative ELISA binding assay against streptavidin.
Endotoxin
Endotoxin levels are less than 10 EU/mg, as determined by the LAL method.
Host cell protein
Host cell protein content is less than 0.5 ng/µg of protein, as tested by ELISA.
Host cell DNA
Host cell DNA content is less than 0.02 ng/μg of protein, as determined by qPCR.
Sterility
Sterility testing is conducted using the membrane filtration method, as outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1.
Mycoplasma
Tested negative for mycoplasma contamination.
Purity
Purity is greater than 95 %, as determined by SDS-PAGE analysis.
Formulation
The product is lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with added protectants.
Shipping
The product is supplied and shipped with blue ice.
Storage
Upon receipt, store the product immediately at -20 °C or lower for long-term preservation. Avoid repeated freeze-thaw cycles.
The product remains stable under the following conditions:
- Lyophilized state: -20 °C to -70 °C for up to 5 years.
- Reconstituted state: -70 °C for up to 12 months under sterile conditions.
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
SDS-PAGE
GMP Biotinylated Human CD5 Protein, His, Avitag was analyzed by SDS-PAGE under both reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The protein exhibits a purity greater than 95 %.
Image Credit: ACROBiosystems