Bone Morphogenetic Protein 4 (BMP4) is a growth factor belonging to the TGF-beta superfamily. It plays a crucial role in various developmental processes, including neurogenesis, vascular development, angiogenesis, and osteogenesis.
BMP4 initiates the canonical BMP signaling cascade by binding to type I receptor BMPR1A and type II receptor BMPR2. Additionally, BMP4 works in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and inhibit hair follicle induction.
Introduction
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Animal-free materials
- Beta-lactam-free formulation
- Consistent batch-to-batch quality
- Stringent quality control testing
- No animal-derived peptone or lactose used in the production process
Source
GMP Human BMP-4 (Improved Sequence) Protein (GMP-BM4H18) is expressed in E. coli cells. This variant of BMP-4 is optimized for cell culture applications.
N-terminus prediction: Met
Molecular characterization
This protein carries no tag.
It has a calculated molecular weight (MW) of 13.2 kDa. When analyzed under reducing (R) conditions in SDS-PAGE, it migrates at 13 kDa ± 3 kDa, as calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
Using the LAL method, less than 50 EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative.
Purity
>95 %, according to SDS-PAGE.
Formulation
Lyophilized in a 0.22 μm filtered solution with protectants in 20 mM citric acid at pH 2.2.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term storage.
Avoid repeated freeze-thaw cycles.
This product remains stable under the following conditions:
- Lyophilized state: -20 °C to -70 °C for 5 years
- After reconstitution (under sterile conditions): -70 °C for 12 months
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Human BMP-4 (Improved Sequence) Protein was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming that the protein's purity exceeds 95 %, as assessed using the Star Ribbon Pre-stained Protein Marker.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems
GMP Human BMP-4 (Improved Sequence) Protein (Cat. No. GMP-BM4H18) stimulates Human BMP (Luc) HEK293 Reporter Cells. The specific activity of GMP Human BMP-4 (Improved Sequence) Protein is >1.90 × 103 U/mg (QC tested).
Application data
Image Credit: ACROBiosystems
GMP Human BMP-4 (Improved Sequence) Protein (Cat. No. GMP-BM4H18) significantly induces iPSC-derived HSPC differentiation, as demonstrated by high CD34 and CD45 expression (HSPC markers) in flow cytometry analysis and an increase in viable CD34+ CD45+ cell numbers by cell counting. Its performance is comparable to Human BMP-4 (Improved Sequence) Protein, Premium Grade (Cat. No. BM4-H5118).
Bioactivity-Stability
Image Credit: ACROBiosystems
The GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) is stable at 37 °C for seven hours, according to the cell-based assay.
Image Credit: ACROBiosystems
The GMP Human BMP-4 (improved sequence) Protein (Cat. No. GMP-BM4H18) is stable after three freezing and thawing cycles, according to the cell-based assay.
Image Credit: ACROBiosystems
Batch-to-batch consistency between ACRO's GMP and PG BMP-4 (improved sequence) is demonstrated by the cell-based assay.