Cations such as ammonium and counter ions of organic acids may be present in tobacco additives. The flavor and moisture of the tobacco is retained using these additive components.
The appeal of smoking is increased with the addition of ammonium and as a result, the addictive potential is also considered to be increased. To determine the presence of cations in tobacco additives, ion chromatographic separation is performed followed by non-suppressed conductivity detection.
Sample
Tobacco additive
Sample preparation
0.1 g additive dissolved in 100 mL of eluent followed by 1:20 dilution with eluent.
Columns
|
Metrosep C 6 - 150/4.0
|
6.1051.420
|
|
Metrosep RP 2 Guard/3.5
|
6.1011.030
|
Solutions
|
Eluent
|
6.25 mmol/L nitric acid
|
Analysis
Direct conductivity detection
Instrumentation
|
940 Professional IC Vario ONE
|
2.940.1100
|
|
IC Conductivity Detector
|
2.850.9010
|
|
919 IC Autosampler plus
|
2.919.0020
|
|
Flow rate
|
0.9 mL/minutes
|
|
Injection volume
|
5 μL
|
|
Pmax
|
25 MPa
|
|
Recording time
|
8.5 minutes
|
|
Column temperature
|
32 °C
|
Results
|
Cation
|
Concentration (mg/g)
|
|
1
|
Lithium
|
n.q.
|
|
2
|
Sodium
|
0.67
|
|
3
|
Ammonium
|
0.42
|
|
4
|
Magnesium
|
32.5
|
|
5
|
Potassium
|
249.3
|
|
6
|
Calcium
|
n.q.
|
About Metrohm
At Metrohm is one of the world’s most trusted manufacturers of high-precision instruments for chemical analysis. Metrohm was founded in 1943 by engineer Bertold Suhner in Herisau, Switzerland. Today, Metrohm is represented in 120 countries by subsidiaries and exclusive distributors. The global Metrohm Group also includes the Dutch companies Metrohm Applikon and Metrohm Autolab, manufacturers of online analyzers and instruments for electrochemical research, respectively. Recently, the Metrohm Group was joined by Metrohm Raman, a leading manufacturer of handheld Raman spectrometers.
Metrohm is the global market leader in analytical instruments for titration. Instruments for ion chromatography, voltammetry, conductivity, and stability measurement make the Metrohm portfolio for ion analysis complete. Instruments for Near-infrared and Raman spectroscopy are another, strongly growing segment of the Metrohm portfolio.
Metrohm is a problem solver, both in the laboratory and within the industrial process. To this end, the company offers their customers complete solutions, including dedicated analytical instrumentation as well as comprehensive application know-how. More than 30% of the company’s employees at the Metrohm international headquarters in Herisau work in R&D.
Metrohm has been owned 100% by the non-profit Metrohm Foundation since 1982. The Metrohm Foundation, which does not exert any influence on the company’s business operations, sponsors gifted students in the natural sciences, supports charitable and philanthropic purposes and, above all, ensures the independence of the company.
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