Orange juice includes inositol along with typical sugar components. Inositol acts as the basis for signaling and messenger molecules. This article discusses the application of pulsed amperometric detection (PAD) to determine inositol as well as glucose, sucrose, and fructose contents in orange juice on a Metrosep Carb 2 - 150/4.0 column.
Sample
Orange juice
Sample preparation
Dilution 1:1000.
Columns
| Metrosep Carb 2 - 150/4.0 |
6.1090.420 |
| Metrosep Carb 2 Guard/4.0 |
6.1090.500 |
Solutions
| Eluent |
100 mmol/L sodium hydroxide
10 mmol/L sodium acetate |
Parameters
| . |
. |
| Flow rate |
0.5 mL/min |
| Injection volume |
20 μL |
| Recording time |
20 minutes |
| Column temperature |
30 °C |
PAD parameters
| Cell |
Wall-Jet cell |
| Working electrode |
Gold |
| Reference electrode |
Palladium |
| Spacer |
50 μm |
| Measuring potential |
0.05 V |
| Measuring duration |
100 ms |
| Cycle duration |
550 ms |
| Measuring range |
200 μA |
| Temperature |
35 °C |
| Mode |
PAD |
Analysis
Pulsed amperometric detection
Instrumentation
| 930 Compact IC Flex Oven/Deg |
2.930.2160 |
| IC Amperometric Detector |
2.850.9110 |
| 858 Professional Sample Processor |
2.858.0020 |
| IC equipment Wall-Jet cell: Carb (Au, Pd) |
6.5337.010 |
Results
| Compound |
Conc. [g/L] |
RSD (%, n = 12) |
| 1 |
Inositol |
1.5 |
0.5 |
| 2 |
Glucose |
20.6 |
0.3 |
| 4 |
Fructose |
23.2 |
0.3 |
| 5 |
Sucrose |
42.5 |
0.4 |
About Metrohm
At Metrohm is one of the world’s most trusted manufacturers of high-precision instruments for chemical analysis. Metrohm was founded in 1943 by engineer Bertold Suhner in Herisau, Switzerland. Today, Metrohm is represented in 120 countries by subsidiaries and exclusive distributors. The global Metrohm Group also includes the Dutch companies Metrohm Applikon and Metrohm Autolab, manufacturers of online analyzers and instruments for electrochemical research, respectively. Recently, the Metrohm Group was joined by Metrohm Raman, a leading manufacturer of handheld Raman spectrometers.
Metrohm is the global market leader in analytical instruments for titration. Instruments for ion chromatography, voltammetry, conductivity, and stability measurement make the Metrohm portfolio for ion analysis complete. Instruments for Near-infrared and Raman spectroscopy are another, strongly growing segment of the Metrohm portfolio.
Metrohm is a problem solver, both in the laboratory and within the industrial process. To this end, the company offers their customers complete solutions, including dedicated analytical instrumentation as well as comprehensive application know-how. More than 30% of the company’s employees at the Metrohm international headquarters in Herisau work in R&D.
Metrohm has been owned 100% by the non-profit Metrohm Foundation since 1982. The Metrohm Foundation, which does not exert any influence on the company’s business operations, sponsors gifted students in the natural sciences, supports charitable and philanthropic purposes and, above all, ensures the independence of the company.
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