Delta-like protein 4 (DLL4), also known as Drosophila Delta homolog 4 (Delta4), contains one DSL domain and eight EGF-like domains. It is expressed in vascular endothelium and functions as a Notch ligand, activating NOTCH1 and NOTCH4. DLL4 plays a role in angiogenesis by negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. It can bind to both Notch-1 and Notch-4.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
Source
GMP Biotinylated Human DLL4 Protein, His, Avitag (GMP-DL4H23) is expressed in human 293 cells (HEK293) and consists of amino acids Ser27–Pro524 (Accession # NP_061947.1).
Predicted N-terminus: Ser 27
Molecular characterization
Image Credit: ACROBiosystems
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
It has a calculated molecular weight of 57.9 kDa but migrates at approximately 61 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE due to glycosylation.
Labeling
Avitag™ technology is used to biotinylate this product. In short, biotin is enzymatically labeled to the Avitag’s single lysine residue.
Biotinylation
As evaluated by a quantitative ELISA binding experiment using streptavidin.
Endotoxin
Less than 10 EU/mg as determined by the LAL method.
Host cell protein
<0.5 ng/µg of protein tested by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Membrane filtration was used for sterility testing, as per CP<1101>, USP<71>, and Eur. Ph. 2.6.1 protocols.
Mycoplasma
Negative
Purity
>95 % as determined by SDS-PAGE.
Formulation
Lyophilized from a 0.22 μm filtered solution in PBS (pH 7.4) with protectants.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term storage.
Avoid repeated freeze-thaw cycles.
This product remains stable under the following conditions:
- -20 °C to -70 °C for 5 years in lyophilized state
- Following reconstitution, store at -70 °C for 12 months under sterile circumstances
ACRO quality management system
- QMS（ISO, GMP)
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Biotinylated Human DLL4 Protein, His, Avitag was analyzed by SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 %.
Bioactivity-SPR
Image Credit: ACROBiosystems
GMP Biotinylated Human DLL4 Protein, His, Avitag (Cat. No. GMP-DL4H23) immobilized on a CM5 chip binds to Human NOTCH1 Protein, Fc Tag (Cat. No. NO1-H5255) with an affinity constant of 1.00 nM – 30.0 nM, as determined by SPR assay (Biacore 8K) (QC tested).
Application data
Image Credit: ACROBiosystems
CD34+ CD45+ hematopoietic cells were seeded on GMP Biotinylated Human DLL4 Protein, His, Avitag (Cat. No. GMP-DL4H23) coated plates and differentiated for 14 days. Flow cytometry analysis detected the expression of T-cell progenitor markers CD7 and CD5. In combination with SCF, TPO, Flt3L, and IL7, the GMP Biotinylated Human DLL4 Protein, His, Avitag induced the formation of CD7+ and CD7+ CD5+ T-cell progenitors (routinely tested).
Bioactivity-Stability
Image Credit: ACROBiosystems
The SPR-based assay demonstrates that GMP Biotinylated Human DLL4 Protein, His, Avitag (Cat. No. GMP-DL4H23) is constant at 37 ℃ for 24 hours.