Interleukin-6 (IL-6), also known as HGF, BSF-2, HSF, IFN-β2, and IL-6, was initially identified as a B-cell differentiation factor. It is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase reactions, and inflammation. IL-6 is secreted by various cell types, including T cells, macrophages, monocytes, fibroblasts, and endothelial cells, primarily in response to trauma—such as burns or tissue damage—triggering inflammation.
IL-6 interacts with the interleukin-6 receptor and glycoproteins, playing a key role in several disease processes, including diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis. Notably, patients with advanced or metastatic cancer often have elevated IL-6 levels in their blood. This has led to significant interest in developing anti-IL-6 therapies to target these conditions.
Features
- Rigorous quality control testing
- Free from beta-lactam materials
- Animal-free materials
- Designed in accordance with ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Ensures batch-to-batch consistency
Source
Human 293 cells (HEK293) express the GMP Human IL-6 Protein (GMP-L06H27). AA Val 30–Met 212 (Accession # P05231) is included.
N-terminus prediction: Val 30
Molecular characterization
Image Credit: ACROBiosystems Inc.
There is no "tag" on this protein.
The protein's estimated molecular weight is 20.8 kDa. Glycosylation causes the protein to migrate as 23–29 kDa when measured against the Star Ribbon Pre-stained Protein Marker under reducing (R) conditions (SDS-PAGE).
Endotoxin
Using the LAL method, less than 10 EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative.
Purity
>95%, according to SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
For long-term storage, store it at -20 °C or lower.
Refrain from repeating freeze-thaw cycles.
After being lyophilized, this product can be stored at
- -20 °C to -70 °C for five years; after reconstitution
- It can be stored at -70 °C for a year under sterile conditions.
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
GMP Human IL-6 Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker). Image Credit: ACROBiosystems Inc.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems Inc.
The TF-1 human erythroleukemic cell line is stimulated to proliferate by GMP Human IL-6 Protein (Cat. No. GMP-L06H27). When calibrated against the human IL-6 WHO International Standard (NIBSC code: 21/308), the specific activity of GMP Human IL-6 Protein is >1.00 × 108 IU/mg (QC tested).
Image Credit: ACROBiosystems Inc.
Compared to competing products, GMP Human IL-6 Protein (Cat. No. GMP-L06H27) exhibited a higher activity level.
Application data
Image Credit: ACROBiosystems Inc.
CD34+ hematopoietic stem cells may benefit from the quick cell division and high cell viability provided by GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18), and GMP Human IL-6 Protein (Cat. No. GMP-L06H27).
Bioactivity-Stability
Image Credit: ACROBiosystems Inc.
According to the cell-based assay, GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc.
According to the cell-based assay, GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable after three freezing and thawing cycles.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates that the GMP Human IL-6 Protein (Cat. No. GMP-L06H27) is stable at 4 °C for six months.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates batch-to-batch consistency between Acro's GMP and PG IL-6.