FMS-like tyrosine kinase 3 ligand (FLT3LG), also known as FL, Flt3L, and FLT3LG, is an α-helical cytokine crucial for the differentiation of multiple hematopoietic cell lineages. It is expressed as a non-covalently linked dimer by T cells, as well as bone marrow and thymic fibroblasts. Each 36 kDa chain contains approximately 12 kDa of N- and O-linked carbohydrates. Structurally, FLT3LG shares homology with stem cell factor (SCF) and colony-stimulating factor 1 (CSF-1).
As a growth factor, FLT3LG enhances immune cell production by activating hematopoietic progenitors and facilitating their mobilization in vivo, potentially aiding the immune system’s ability to target cancer cells. It also promotes the expansion of monocytes and immature dendritic cells while supporting early B cell lineage differentiation.
FLT3LG functions alongside IL-2, IL-6, IL-7, and IL-15 to drive NK cell development and works with IL-3, IL-7, and IL-11 to promote terminal B cell maturation. Animal studies suggest FLT3LG may help reduce the severity of experimentally induced allergic inflammation. Additionally, FLT3LG is essential for the development of steady-state plasmacytoid (pDC) and conventional dendritic cells (cDC), as its absence leads to significantly reduced dendritic cell (DC) levels.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- FDA DMF filed
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
Source
GMP Human Flt-3 Ligand Protein (GMP-FLLH28) is expressed in human 293 cells (HEK293) and consists of amino acids Thr27–Pro185 (Accession # P49771-1).
N-terminus prediction: Thr 27
Molecular characterization
Image Credit: ACROBiosystems
This protein carries no tag.
It has a calculated molecular weight of 18.0 kDa but migrates at approximately 24 kDa and 27 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE due to glycosylation.
Endotoxins
The LAL method yielded less than 10 EU/mg.
Host cell protein
The host cell protein was measured by ELISA and was less than 0.5 ng/µg.
Host cell DNA
qPCR-tested host cell DNA <0.02 ng/μg of protein.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative
Purity
>95 %, according to SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
For long-term storage, store it at -20 °C or lower as soon as people receive it.
Please avoid repeated freeze-thaw cycles.
Once stored, this product is stable at:
- In a lyophilized state, -20 °C to -70 °C for 5 years
- After reconstitution, -70 °C for 12 months under sterile conditions.
ACRO quality management system
- The ISO, GMP, and QMS
- Quality Advantages
- Process of Quality Control
QC and assay protocol
SDS-PAGE
GMP Human Flt-3 Ligand Protein was analyzed by SDS-PAGE under reducing (R) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 %.
Image Credit: ACROBiosystems
Bioactivity-Bioactivity CELL BASE
GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) stimulates the proliferation of OCI-AML5 cells. Its specific activity is >5.00 × 105 IU/mg, calibrated against the WHO Reference Reagent FMS-Like Tyrosine Kinase 3 Ligand (NIBSC code: 96/532) (QC tested).
Image Credit: ACROBiosystems
The activity of GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) was higher than other competing products.
Image Credit: ACROBiosystems
Application data
GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF Basic Protein (Cat. No. GMP-FGCH17), and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) significantly promote iPSC differentiation into HSPCs after 14 days, resulting in high expression of hematopoietic stem cell markers CD34 and CD45.
Image Credit: ACROBiosystems
GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18), and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) support the rapid expansion and maintain the viability of CD34+ hematopoietic stem cells.
Image Credit: ACROBiosystems
Bioactivity-Stability
The cell-based assay shows that GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems
The cell-based assay shows that GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) is stable after freezing and thawing 3 times.
Image Credit: ACROBiosystems
According to the cell-based assay, the GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) is stable at 4 °C for 180 days.
Image Credit: ACROBiosystems
Batch-to-batch consistency between PG Flt-3 Ligand and Acro's GMP is demonstrated by the cell-based assay.
Image Credit: ACROBiosystems