Interleukin-18 (IL-18) is a powerful proinflammatory cytokine that stimulates interferon-gamma (IFN-γ) production from Th1 cells, natural killer (NK) cells, and activated macrophages, especially in the presence of IL-12. Beyond its role in immune activation, IL-18 contributes to the developmental regulation of T-helper type 1 (Th1) cells and plays a part in Fas-mediated cytotoxicity. Given its involvement in inflammatory pathways, researchers are exploring IL-18 suppression as a potential treatment for chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. IL-18 exerts its effects by promoting the heterodimerization of its receptor subunits, IL-18Rα and IL-18Rβ, and shares structural similarities with IL-1.
Features
- Developed in compliance with ISO 9001:2015 and ISO 13485:2016 standards
- Produced and quality control (QC) tested in a GMP-compliant facility
- Composed of animal-free materials
- Free from beta-lactam components
- Ensures batch-to-batch consistency
- Undergoes rigorous quality control testing
- No animal-derived peptone or lactose is used during the production process
Source
GMP Human IL-18 Protein (GMP-L18H16) is expressed in E. coli cells. It includes amino acids Tyr 37 to Asp 193.
Predicted N-terminus: Tyr 37
Molecular characterization
Image Credit: ACROBiosystems Inc
- This protein is tag-free.
- The calculated molecular weight (MW) is 18.2 kDa. When analyzed under reducing conditions (SDS-PAGE) and compared to the Star Ribbon Pre-stained Protein Marker, the protein migrates as 17 kDa ± 3 kDa.
Endotoxin
Contains less than 10 EU/mg as determined by the LAL method.
Host cell protein
Contains less than 0.5 ng/µg of protein, as tested by ELISA.
Host cell DNA
Contains less than 0.02 ng/μg of protein, as tested by qPCR.
Sterility
Sterility testing uses membrane filtration, as outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1.
Mycoplasma
Tested negative for mycoplasma contamination.
Purity
Purity exceeds 95%, as determined by SDS-PAGE.
Formulation
- Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with added protectants.
Shipping
The product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at –20 °C or lower for long-term preservation.
Avoid repeated freeze-thaw cycles.
Stability:
- Lyophilized state: Stable for 5 years when stored at -20 °C to -70 °C.
- After reconstitution: Stable for 12 months under sterile conditions at -70 °C.
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Ensures a robust quality control process.
QC and assay protocol
SDS-PAGE
SDS-PAGE analysis of GMP Human IL-18 Protein in reducing (R) and non-reducing (NR) conditions. Coomassie Blue was used to stain the gel. According to Star Ribbon Pre-stained Protein Marker, the protein's purity is higher than 95%.
Image Credit: ACROBiosystems Inc
Bioactivity-Bioactivity CELL BASE
GMP Human IL-18 Protein (Cat. No. GMP-L18H16) causes KG-1 cells to secrete more IFN-γ. The WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) is used to calibrate the specific activity of GMP Human IL-18 Protein, which is > 3.00ⅹ106 IU/mg (QC tested).
Image Credit: ACROBiosystems Inc
Compared to competing products, GMP Human IL-18 Protein (Cat. No. GMP-L18H16) exhibited a higher level of activity.
Image Credit: ACROBiosystems Inc
Application data
For two weeks, human PBMCs were cultivated in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16), and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26).
The outcome demonstrates that GMP Human IL-18 Protein can encourage these cells' growth while maintaining a respectable level of cell viability. The cells notably show improved GMP Human IL-18 Protein (ACROBiosystems) growth.
Image Credit: ACROBiosystems Inc
For two weeks, human PBMCs were cultivated in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16), and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26).
The outcome demonstrates that the competitor M IL-18 protein and GMP Human IL-18 Protein (ACROBiosystems) can be compared.
Image Credit: ACROBiosystems Inc
Bioactivity-Stability
The cell-based assay demonstrates batch-to-batch consistency between Acro's GMP and PG IL-18.
Image Credit: ACROBiosystems Inc