FGF-8 is a member of the fibroblast growth factor family, originally identified as essential for the androgen-dependent growth of mouse mammary carcinoma cells.
In mice, alternative splicing of the FGF8 mRNA produces eight different secreted isoforms (a–h), whereas humans generate only four variants (FGF-8a, b, e, and f). The FGF-8 protein includes a 22-amino acid (aa) signal sequence, an isoform-specific N-terminal region (30 aa in FGF-8b and 20 aa in FGF-8a, the shortest form), a 125-aa FGF domain, and a 37-aa proline-rich C-terminal sequence. The FGF domain shares 75 % sequence identity with FGF-17 and 67 % with FGF-18, grouping them into the same FGF subfamily. Interestingly, mouse FGF-8b is completely identical to its human equivalent at the amino acid level.
During embryogenesis, FGF-8 is broadly expressed, facilitating epithelial-mesenchymal transitions and playing a crucial role in gastrulation. It is essential for midbrain/hindbrain patterning and contributes to the development of the eyes, ears, limbs, and heart. The various FGF-8 isoforms may have distinct biological functions, with FGF-8b exhibiting the strongest receptor affinity and the highest oncogenic potential. However, FGF-8a and FGF-8e also possess transforming properties and have been identified in prostate, breast, and ovarian tumors.
In healthy adult tissues, FGF-8 expression is generally restricted but can be detected at low levels in the reproductive and genitourinary tract, peripheral leukocytes, and bone marrow hematopoietic cells.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
- No animal-derived peptone or lactose used in the production process
Source
GMP Human FGF-8b Protein (Cat. No. GMP-FGBH16) is expressed in E. coli cells and consists of amino acids Gln23–Arg215 (Accession # P55075-3).
Predicted N-terminus: Met
Molecular characterization
Image Credit: ACROBiosystems
This protein carries no tag.
It has a calculated molecular weight of 22.5 kDa but migrates at approximately 25 kDa ±3 kDa under reducing (R) conditions in SDS-PAGE when calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
The LAL technique yields less than ten EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative
Purity
>95 % as determined by SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term storage.
Avoid repeated freeze-thaw cycles.
This product remains stable under the following conditions:
- -20 °C to -70 °C for 5 years in lyophilized state;
- -70 °C for 12 months under sterile conditions after reconstitution
ACRO quality management system
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Human FGF-8b Protein was analyzed by SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 % when calibrated against the Star Ribbon Pre-stained Protein Marker.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems
GMP Human FGF-8b Protein (Cat. No. GMP-FGBH16) stimulates the proliferation of NIH-3T3 cells. Its specific activity is >2.00 × 103 U/mg (QC tested).
Application data
The mRNA expression of pluripotent stem cell markers (Oct4, Nanog) was higher in Acro than in company R and company P. Right: When FGF basic (35 μg/mL) is added into the culture media (StemSure hPSC medium) for iPSC, the cells remain high expression of pluripotent (Oct4+ or Nanog+; pink) markers. Image Credit: ACROBiosystems
GMP Human FGF-8b Protein (Cat. No. GMP-FGBH16) and Human Sonic Hedgehog, His Tag, Premium Grade (Cat. No. SH7-H5229) efficiently induce the differentiation of neuronal progenitor cells into dopaminergic neurons, as demonstrated by high expression of TH1 and MAP2 in immunofluorescence staining and FACS (routinely tested).
Image Credit: ACROBiosystems
GMP Human FGF-8b Protein (Cat. No. GMP-FGBH16) and Human FGF-8b Protein, Premium Grade (Cat. No. FGB-H5115) exhibit similar bioactivity in efficiently inducing neuronal progenitor cell differentiation into dopaminergic neurons, as shown by high expression of TH1 and MAP2 in immunofluorescence staining and FACS (routinely tested).
Bioactivity-Stability
Image Credit: ACROBiosystems
The cell-based assay demonstrates batch-to-batch consistency between Acro's GMP and PG FGF-8b.