Interleukin-3 (IL-3) is a human protein encoded by the IL3 gene. IL-3 plays a key role in the immune system as a cytokine, enhancing the body's natural response to disease. It exerts its effects by binding to the interleukin-3 receptor, which consists of a specific α-chain and a shared β-chain (βc). GM-CSF and IL-5 also utilize the latter. In some cases, IL-3 can bind to an alternative βIL-3 chain, which appears unique to IL-3.
Upon receptor binding, IL-3 activates Jak2, phosphorylating multiple Stat proteins and activating the PI3K/Akt signaling pathway. This signaling cascade contributes to airway inflammation, particularly in asthma.
The human IL3 gene is located on chromosome 5, just 9 kilobases from the GM-CSF gene, reflecting its functional similarity to GM-CSF. The IL-3 protein is 152 amino acids long and naturally glycosylated.
Features
- No animal-derived peptone or lactose used in the production process
- Ensures batch-to-batch consistency
- Designed in accordance with ISO 9001:2015 and ISO 13485:2016 standards
- Free from beta-lactam materials
- Manufactured and QC tested in a GMP-compliant facility
- Animal-free materials
- Subjected to stringent quality control tests
Source
E. Coli cells express GMP Human IL-3 Protein (GMP-L03H18). AA Ala 20–Phe 152 (Accession # P08700-1) is present.
Predicted N-terminus: Met
Molecular characterization
Image Credit: ACROBiosystems Inc.
There is no "tag" on this protein.
The protein's estimated molecular weight is 15.1 kDa. When calibrated against the Star Ribbon Pre-stained Protein Marker under reducing (R) conditions (SDS-PAGE), the protein migrates as 14 kDa ± 3 kDa.
Endotoxin
Using the LAL method, less than 10 EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative.
Purity
>95%, according to SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term preservation.
Avoid repeated freeze-thaw cycles.
Following storage, this product remains stable at:
- -70 °C for 12 months under sterile conditions after reconstitution.
- -20 °C to -70 °C for 5 years in lyophilized state.
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc.
SDS-PAGE analysis of GMP Human IL-3 Protein in both reducing (R) and non-reducing (NR) conditions. Coomassie Blue was used to stain the gel. With the Star Ribbon Pre-stained Protein Marker, the protein's purity is higher than 95%.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems Inc.
TF-1 cell proliferation is stimulated by GMP Human IL-3 Protein (Cat. No. GMP-L03H18). When compared to the human IL-3 WHO International Standard (NIBSC code: 91/510) (QC tested), the specific activity of GMP Human IL-3 Protein is >1.00 × 106 IU/mg.
Image Credit: ACROBiosystems Inc.
Compared to other competing products, GMP Human IL-3 (Cat. No. GMP-L03H18) had a higher activity level.
Application data
Image Credit: ACROBiosystems Inc.
CD34+ hematopoietic stem cells may benefit from the quick cell division and high cell viability provided by GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18), and GMP Human IL-6 Protein (Cat. No. GMP-L06H27).
Bioactivity-Stability
Image Credit: ACROBiosystems Inc.
According to the cell-based assay, GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates that the GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 4 °C for 180 days.
Image Credit: ACROBiosystems Inc.
GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable after three freezing and thawing cycles, according to the cell-based assay.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates batch-to-batch consistency between Acro's GMP and PG IL-3.