Fibronectin (Fn) is a glycoprotein ranging from 230 to 270 kDa, typically existing as a dimer linked by disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15–17 Type III domains, collectively making up about 90 % of the Fn sequence.
The extracellular matrix (ECM) functions as both a structural scaffold and a regulator of cell signal transduction within tissues. Fibronectin plays a crucial role in the trabecular meshwork (TM), where it is found in the sheath material surrounding elastin tendons that extend from the ciliary muscle within the ciliary body.
During ECM assembly and turnover, cells increase Fn production, integrating it into viscoelastic fibrils capable of binding more than 40 distinct growth factors and cytokines. These fibrils are essential for provisional ECM formation in embryonic development and wound healing. Additionally, fibril assembly is upregulated in pathological conditions such as cancer and fibrotic diseases.
Features
- Designed under ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Made with animal-free materials
- Free from beta-lactam materials
- Ensures batch-to-batch consistency
- Subjected to stringent quality control tests
- No animal-derived peptone or lactose used in the production process
Source
GMP Recombinant Human Fibronectin Fragment (GMP-FINH18) is expressed in E. coli cells and consists of amino acids Pro1361–Ser1637 & Ala1812–Thr2107 (Accession # P02751-15).
Predicted N-terminus: Met
Molecular characterization
Image Credit: ACROBiosystems
This protein carries no “tags.”
The protein's molecular weight is estimated to be 62.6 kDa. Under reducing (R) conditions (SDS-PAGE), the protein migrates as 60 kDa ± 3 kDa when calibrated against the Star Ribbon Pre-stained Protein Marker.
Endotoxin
Using the LAL method, less than 10 EU/mg.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasm
Negative
Purity
>95 %, according to SDS-PAGE
>90 %, according to SEC-HPLC
Formulation
Supplied in a 0.2 μm filtered solution with protectants in 12.5 mM citric acid at pH 6.2.
Shipping
This product is supplied and shipped with dry ice.
Storage
Avoid repeated freeze-thaw cycles.
Following storage, this product remains stable at:
- -20 °C ± 10 °C for three years in a sterile environment.
ACRO quality management system
- QMS（ISO, GMP）ISO, GMP, and QMS
- Quality Benefits
- Procedure for Quality Control
QC and assay protocol
SDS-PAGE
GMP Recombinant Human Fibronectin Fragment was analyzed by SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue, confirming a purity of greater than 95 % when calibrated against the Star Ribbon Pre-stained Protein Marker.
Image Credit: ACROBiosystems
SEC-HPLC
The purity of GMP Recombinant Human Fibronectin Fragment (Cat. No. GMP-FINH18) was greater than 90 %, as determined by SEC-HPLC.
Image Credit: ACROBiosystems
Application data
GMP Recombinant Human Fibronectin Fragment (Cat. No. GMP-FINH18) promotes mesenchymal stem cell attachment and proliferation across multiple passages, showing comparable performance to Competitor T. Its bioactivity is similar to Recombinant Fibronectin Fragment, Premium Grade (Cat. No. FIN-H5116).
Image Credit: ACROBiosystems
GMP Recombinant Human Fibronectin Fragment (Cat. No. GMP-FINH18) supports the maintenance of MSC stemness across multiple passages, ensuring high expression of MSC markers CD73, CD90, and CD105, while maintaining negative expression of HLA-DR, CD34, and CD45. Its stemness-supporting ability is comparable to Competitor T and similar to Recombinant Fibronectin Fragment, Premium Grade (Cat. No. FIN-H5116).
Image Credit: ACROBiosystems