Interleukin-4 (IL-4) is a cytokine differentiating naive helper T cells (Th0) into Th2 cells. Along with IL-13, IL-4 plays a key role in Th2-type immune responses, particularly in allergic reactions and parasitic infections. In the presence of these cytokines, macrophages undergo differential activation.
IL-4 is a ligand for the interleukin-4 receptor (IL-4R), which also binds IL-13, leading to overlapping functions between these two cytokines. The signal transducer and activator of transcription 6 (STAT6) has been identified as a crucial mediator of IL-4’s immune regulatory effects.
Recent research has highlighted IL-4’s role in developing innate CD8+ T cells in the thymus, particularly in gene-deficient mouse models lacking Itk, KLF2, CBP, and Id3. This process occurs without prior antigen exposure, suggesting IL-4’s involvement in shaping innate immune responses.
Features
- Rigorous quality control testing
- Free from beta-lactam materials
- Animal-free materials
- Designed in accordance with ISO 9001:2015 and ISO 13485:2016 standards
- Manufactured and QC tested in a GMP-compliant facility
- Ensures batch-to-batch consistency
Source
Human 293 cells (HEK293) express the GMP Human IL-4 Protein (GMP-L04H26). AA His 25-Ser 153 (Accession # P05112-1) is included in it.
Predicted N-terminus: His 25
Molecular characterization
Image Credit: ACROBiosystems Inc.
There is no "tag" on this protein.
The protein's estimated molecular weight is 15.0 kDa. Glycosylation causes the protein to migrate as 19 kDa ±3 kDa under reducing (R) conditions (SDS-PAGE).
Endotoxin
Less than 10 EU/mg using the LAL method
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
qPCR-tested protein at less than 0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative.
Purity
SDS-PAGE shows >95%
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
This product is supplied and shipped with blue ice.
Storage
Upon receipt, store immediately at -20 °C or lower for long-term preservation.
Avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -70 °C for 12 months under sterile conditions after reconstitution.
- -20 °C to -70 °C for five years in lyophilized state.
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc.
GMP Human IL-4 Protein in reducing (R) conditions on SDS-PAGE. Coomassie Blue was used to stain the gel. The protein has a purity of over 95%.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems Inc.
The TF-1 human erythroleukemic cell line is stimulated to proliferate by GMP Human IL-4 Protein (Cat. No. GMP-L04H26). When compared to the human IL-4 WHO International Standard (NIBSC code: 88/656), the specific activity of GMP Human IL-4 Protein is >1.20 × 107 IU/mg (QC tested).
Image Credit: ACROBiosystems Inc.
Compared to competing products, GMP Human IL-4 Protein (Cat. No. GMP-L04H26) exhibited a higher level of activity.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc.
The GMP Human IL-4 Protein (Cat. No. GMP-L04H26) is stable at 37 °C for 24 hours, according to the cell-based assay.
Image Credit: ACROBiosystems Inc.
According to the cell-based assay, the GMP Human IL-4 Protein (Cat. No. GMP-L04H26) is stable at 4 °C for 180 days.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates that the GMP Human IL-4 Protein (Cat. No. GMP-L04H26) is stable after three freezing and thawing cycles.
Image Credit: ACROBiosystems Inc.
Batch-to-batch consistency between Acro's GMP and PG IL-4 is demonstrated by the cell-based assay.