VEGF165 is the most abundant splice variant of VEGF-A and is produced by various cell types, including endothelial cells, macrophages, and T cells. It plays a critical role in angiogenesis, promoting vascular endothelial cell survival, growth, migration, and permeability.
Hypoxia, inflammatory cytokines, and oncogenes regulate VEGF expression. VEGF165 specifically binds to heparan sulfate, allowing it to be retained on the cell surface and within the extracellular matrix. It interacts with the receptor tyrosine kinases VEGFR1 and VEGFR2, as well as co-receptors NRP-1 and NRP-2, which enhance VEGFR2 signaling.
VEGF165 activates VEGFR1 and VEGFR2 and triggers key signaling pathways, including PI3K/AKT, p38 MAPK, FAK, and paxillin. These pathways contribute to VEGF’s crucial role in tumor angiogenesis across various cancers.
Features
- Designed in accordance with ISO 13485:2016 and ISO 9001:2015
- Produced and quality-checked in a factory that complies with GMP
- Animal-free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Strict quality control examinations
Source
Human 293 cells (HEK293) express the GMP Human VEGF165 Protein (GMP-VE5H23). AA Ala 27–Arg 191 (Accession # P15692-4) is present.
Predicted N-terminus: Ala 27
Molecular characterization
Image Credit: ACROBiosystems
There is no “tag” on this protein.
The protein’s estimated MW is 19.2 kDa. When measured against the Star Ribbon Pre-stained Protein Marker under reducing (R) conditions (SDS-PAGE), the protein migrates as 24 kDa ± 3 kDa as a result of glycosylation.
Endotoxin
The LAL technique yields less than 10 EU/mg.
Host cell protein
<0.5 ng/µg of protein tested by ELISA.
Host cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Membrane filtration was used for sterility testing, as per CP<1101>, USP<71>, and Eur. Ph. 2.6.1 protocols.
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized in PBS at pH 7.4 with protectants from a 0.22 μm filtered solution.
Shipping
Blue ice is used to package and ship this product.
Storage
When received, store it at –20 °C or lower for long-term storage.
Avoid repeating the freeze-thaw cycle.
This product remains stable after storage at:
- –20 °C to –70 °C for 5 years in lyophilized state
- –70 °C for 12 months under sterile conditions after reconstitution
ACRO quality management system
- QMS（ISO, GMP)
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems
GMP Human VEGF165 Protein on SDS-PAGE under reducing conditions. The gel was stained with Coomassie Blue. The protein purity is more than 95% (using the Star Ribbon Pre-stained Protein Marker).
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems
The GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) promotes 293F-NFAT/Luc-VEGFR2-3-7 cell growth. The human vascular endothelial growth factor 165 WHO International Standard (NIBSC code: 02/286) is used to calibrate the specific activity of GMP Human VEGF165 Protein, which is ＞8.00 × 105 IU/mg (QC tested).
Image Credit: ACROBiosystems
Compared to rival products, GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) exhibited greater activity.
Application data
Image Credit: ACROBiosystems
After 14 days, the highly expressed hematopoietic stem cell markers CD34 and CD45, as well as the GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF basic Protein (Cat. No. GMP-FGCH17), and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23), could significantly promote the iPSC differentiation to HSPCSs.
Bioactivity-Stability
Image Credit: ACROBiosystems
According to the cell-based assay, the GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) is stable at 37 °C for 24 hours.
Image Credit: ACROBiosystems
The GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) is stable following three cycles of freezing and thawing, according to the cell-based experiment.
Image Credit: ACROBiosystems
The cell-based assay demonstrates the GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) is stable at 4 °C for 180 days.
Image Credit: ACROBiosystems
Batch-to-batch consistency between PG VEGF165 and Acro's GMP is demonstrated by the cell-based assay.