Integrin-associated protein (IAP), CD47, was first found in research that focused on integrin αvβ3. This research found that CD47 was to be associated in cis with integrin αvβ3 on the cell membrane.
Further research has found that CD47 is the archetypal “marker of self” that is found on all cells. It plays a key role in the regulation of tissue homeostasis and is involved in a range of diseases that include cancer and atherosclerosis.
CD47 protects host cells by the suppression of phagocytosis. It is already known that the CD47 expressed by cancer cells interacts with NK cell-expressed proteins in the SIRP family – namely SIRP-a and SIRP-g. The interaction protects cancerous cells from being identified and removed via phagocytosis (the innate immune response).
CD47 has also been found to play an essential role in the vascular NO signaling that is mediated by TSP-1. In this role CD47 inhibits angiogenesis via the regulation of CD36 and VEGFR2. Long term dysregulation of NO signaling is indicative of a range of cardiovascular diseases; making CD47 a potential target for atherosclerosis treatment. To this effect, Anti-CD47 mAb has been shown to reduce the amount of atherosclerosis in multiple in vivo experiments.
CD47 Proteins
Given the key role that CD47 plays in the pathogenesis of cardiovascular diseases and cancer it is hardly surprising that significant effort is going into research of CD47 targeted treatments.
ACROBiosystems can supply researchers with a unique range of CD47-relevant products. These include multiple avi tag pre-biotinylated proteins, which can be used for fast, high-throughput screening.
The following product pairs can be easily used for ELISA, MSD platforms and Alphalisa, for the screening of neutralizing compounds.
| Pairs |
Cat. No. |
Product Description |
Structure |
| Pair 1 |
CD7-H82F6 |
Biotinylated Human CD47, Fc Tag, Avi Tag |
 |
| Pair 1 |
SIA-H5225 |
Human SIRP alpha (HPLC-verified) |
 |
| Pair 2 |
CD7-H82E9 |
Biotinylated Human CD47, Avi Tag |
 |
| Pair 2 |
SIA-H5251 |
Human SIRP alpha, Fc Tag (HPLC-verified) |
 |
| Pair 3 |
CD7-H5227 |
Human CD47 Protein |
 |
| Pair 3 |
CDA-H82F2 |
Biotinylated Human SIRP alpha, Fc, Avi Tag |
 |
| |
EP-102 |
CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair |
|
ACROBiosystems provides Fc tagged CD47 and SIRP alpha in mouse models, for use in instances where the human model Fc tagged CD47 could interfere with the assay. These products are uniquely designed to bind with humanized antibodies.
| Molecule |
Cat. No. |
Product Description |
Structure |
| CD47 |
CD7-H52A5 |
Human CD47, mouse IgG2a Fc Tag, low endotoxin |
 |
| SIRP alpha |
SIA-H52A8 |
Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) |
 |
ACROBiosystems also provides CD47 sourced from other commonly used animal models.
References
- Piccio L, et al., 2005, Blood. 105:2421-2427.
- Murata Y, et al., 2014, J Biochem. 155(6):335-344.
- Zhang L, et al., 2016, Anticancer Agents Med Chem. 16(6):658-667.
About ACROBiosystems
ACROBiosystems is a cornerstone enterprise of the pharmaceutical and biotechnology industries. Their mission is to help overcome challenges with innovative tools and solutions from discovery to the clinic. They supply life science tools designed to be used in discovery research and scalable to the clinical phase and beyond. By consistently adapting to new regulatory challenges and guidelines, ACROBiosystems delivers solutions, whether it comes through recombinant proteins, antibodies, assay kits, GMP-grade reagents, or custom services. ACROBiosystems empower scientists and engineers dedicated towards innovation to simplify and accelerate the development of new, better, and more affordable medicine.
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